VE-cadherin junction dynamics in initial lymphatic vessels promotes lymph node metastasis

Sáinz-Jaspeado, Miguel (author): Uppsala universitet,Science for Life Laboratory, SciLifeLab,Vaskulärbiologi

Ring, Sarah (author): Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab

Proulx, Steven T. (author): Swiss Fed Inst Technol, Inst Pharmaceut Sci, Zurich, Switzerland.;Univ Bern, Theodor Kocher Inst, Bern, Switzerland.

Richards, Mark (author): Uppsala universitet,Science for Life Laboratory, SciLifeLab,Vaskulärbiologi

Martinsson, Pernilla (author): Uppsala universitet,Science for Life Laboratory, SciLifeLab,Vaskulärbiologi

Li, Xiujuan (author): Soochow Univ, Cyrus Tang Hematol Ctr, Collaborat Innovat Ctr Hematol, State Key Lab Radiat Med & Protect, Suzhou, Peoples R China.

Claesson-Welsh, Lena (author): Uppsala universitet,Science for Life Laboratory, SciLifeLab,Vaskulärbiologi

Ulvmar, Maria H., Senior Lecturer, 1974- (author): Uppsala universitet,Science for Life Laboratory, SciLifeLab,Institutionen för medicinsk biokemi och mikrobiologi,Vaskulärbiologi

Jin, Yi (author): Uppsala universitet,Science for Life Laboratory, SciLifeLab,Vaskulärbiologi

(creator_code:org_t)

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Abstract Subject headings

The endothelial junction component vascular endothelial (VE)–cadherin governs junctional dynamics in the blood and lymphatic vasculature. Here, we explored how lymphatic junction stability is modulated by elevated VEGFA signaling to facilitate metastasis to sentinel lymph nodes. Zippering of VE-cadherin junctions was established in dermal initial lymphatic vessels after VEGFA injection and in tumor-proximal lymphatics in mice. Shape analysis of pan-cellular VE-cadherin fragments revealed that junctional zippering was accompanied by accumulation of small round-shaped VE-cadherin fragments in the lymphatic endothelium. In mice expressing a mutant VEGFR2 lacking the Y949 phosphosite (Vegfr2Y949F/Y949F) required for activation of Src family kinases, zippering of lymphatic junctions persisted, whereas accumulation of small VE-cadherin fragments was suppressed. Moreover, tumor cell entry into initial lymphatic vessels and subsequent metastatic spread to lymph nodes was reduced in mutant mice compared with WT, after challenge with B16F10 melanoma or EO771 breast cancer. We conclude that VEGFA mediates zippering of VE-cadherin junctions in initial lymphatics. Zippering is accompanied by increased VE-cadherin fragmentation through VEGFA-induced Src kinase activation, correlating with tumor dissemination to sentinel lymph nodes.

By the author/editor: Sáinz-Jaspeado, ...; Ring, Sarah; Proulx, Steven T ...; Richards, Mark; Martinsson, Pern ...; Li, Xiujuan; show more...; Claesson-Welsh, ...; Ulvmar, Maria H. ...; Jin, Yi; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Cell and Molecul ...

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