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The RNA-binding pro...
The RNA-binding protein ZC3H11A interacts with the nuclear poly(A)-binding protein PABPN1 and alters polyadenylation of viral transcripts
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- Kases, Katharina (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Schubert, Erik (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Hajikhezri, Zamaneh (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Larsson, Mårten (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Devi, Priya (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Darweesh, Mahmoud (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Al Azhr Univ, Dept Microbiol & Immunol, Assiut, Egypt.
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- Andersson, Leif (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Akusjärvi, Göran, 1953- (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Punga, Tanel, PhD, 1974- (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Younis, Shady (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi,Stanford Univ, Div Immunol & Rheumatol, Stanford, CA 94305 USA.
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(creator_code:org_t)
- Elsevier, 2023
- 2023
- English.
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In: Journal of Biological Chemistry. - : Elsevier. - 0021-9258 .- 1083-351X. ; 299:8
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Abstract
Subject headings
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- Nuclear mRNA metabolism is regulated by multiple proteins, which either directly bind to RNA or form multiprotein complexes. The RNA-binding protein ZC3H11A is involved in nuclear mRNA export, NF-kappa B signaling, and is essential during mouse embryo development. Furthermore, previous studies have shown that ZC3H11A is important for nuclear-replicating viruses. However, detailed biochemical characterization of the ZC3H11A protein has been lacking. In this study, we established the ZC3H11A protein interactome in human and mouse cells. We demonstrate that the nuclear poly(A)-binding protein PABPN1 interacts specifically with the ZC3H11A protein and controls ZC3H11A localization into nuclear speckles. We report that ZC3H11A specifically interacts with the human adenovirus type 5 (HAdV-5) capsid mRNA in a PABPN1dependent manner. Notably, ZC3H11A uses the same zinc finger motifs to interact with PABPN1 and viral mRNA. Further, we demonstrate that the lack of ZC3H11A alters the polyadenylation of HAdV-5 capsid mRNA. Taken together, our results suggest that the ZC3H11A protein may act as a novel regulator of polyadenylation of nuclear mRNA.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Kases, Katharina
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Schubert, Erik
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Hajikhezri, Zama ...
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Larsson, Mårten
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Devi, Priya
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Darweesh, Mahmou ...
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show more...
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Andersson, Leif
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Akusjärvi, Göran ...
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Punga, Tanel, Ph ...
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Younis, Shady
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Medical Genetics
- Articles in the publication
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Journal of Biolo ...
- By the university
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Uppsala University