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The adenovirus E4-O...
The adenovirus E4-ORF4 splicing enhancer protein interacts with a subsetof phosphorylated SR proteins
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- Estmer Nilsson, Camilla (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Petersen-Mahrt, Svend (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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Durot, C (author)
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Shtrichman, R (author)
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Krainer, AR (author)
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Kleinberger, T (author)
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- Akusjärvi, Göran (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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(creator_code:org_t)
- 2001
- 2001
- English.
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In: EMBO Journal. - 0261-4189 .- 1460-2075. ; 20, s. 864-871
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- SR proteins purified from uninfected HeLa cells inhibit adenovirus IIIa pre-mRNA splicing by binding to the intronic IIIa repressor element (3RE). In contrast, SR proteins purified from late adenovirus-infected cells are functionally inactivated as splicing repressor proteins by a virus-induced dephosphorylation. We have shown that the adenovirus E4-ORF4 protein, which binds the cellular protein phos phatase 2A (PP2A) and activates IIIa splicing in vitro and in vivo, induces SR protein dephosphorylation. Here we show that E4-ORF4 interacts with only a subset of SR proteins present in HeLa cells. Thus, E4-ORF4 interacts efficiently with SF2/ASF and SRp30c, but not with other SR proteins. Interestingly, E4-ORF4 interacts with SF2/ASF through the latter's RNA recognition motifs. Furthermore, E4-ORF4 interacts preferentially with the hyperphosphorylated form of SR proteins found in uninfected HeLa cells. E4-ORF4 mutant proteins that fail to bind strongly to PP2A or SF2/ASF do not relieve the repressive effect of HeLa SR proteins on IIIa pre-mRNA splicing in transient transfection experiments, suggesting that an interaction between all three proteins is required for E4-ORF4-induced SR protein dephosphorylation
Keyword
- adenovirus
- E4-ORF protein
- protein phosphatase 2A
- splicing enhancer
- SR proteins
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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