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The adenovirus E4-O...
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Estmer Nilsson, CamillaUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
(author)
The adenovirus E4-ORF4 splicing enhancer protein interacts with a subsetof phosphorylated SR proteins
- Article/chapterEnglish2001
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Numbers
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LIBRIS-ID:oai:DiVA.org:uu-52465
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-52465URI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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SR proteins purified from uninfected HeLa cells inhibit adenovirus IIIa pre-mRNA splicing by binding to the intronic IIIa repressor element (3RE). In contrast, SR proteins purified from late adenovirus-infected cells are functionally inactivated as splicing repressor proteins by a virus-induced dephosphorylation. We have shown that the adenovirus E4-ORF4 protein, which binds the cellular protein phos phatase 2A (PP2A) and activates IIIa splicing in vitro and in vivo, induces SR protein dephosphorylation. Here we show that E4-ORF4 interacts with only a subset of SR proteins present in HeLa cells. Thus, E4-ORF4 interacts efficiently with SF2/ASF and SRp30c, but not with other SR proteins. Interestingly, E4-ORF4 interacts with SF2/ASF through the latter's RNA recognition motifs. Furthermore, E4-ORF4 interacts preferentially with the hyperphosphorylated form of SR proteins found in uninfected HeLa cells. E4-ORF4 mutant proteins that fail to bind strongly to PP2A or SF2/ASF do not relieve the repressive effect of HeLa SR proteins on IIIa pre-mRNA splicing in transient transfection experiments, suggesting that an interaction between all three proteins is required for E4-ORF4-induced SR protein dephosphorylation
Subject headings and genre
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adenovirus
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E4-ORF protein
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protein phosphatase 2A
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splicing enhancer
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SR proteins
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MEDICINE
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MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
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Petersen-Mahrt, SvendUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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Durot, C
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Shtrichman, R
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Krainer, AR
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Kleinberger, T
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Akusjärvi, GöranUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)gak30132
(author)
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Uppsala universitetInstitutionen för medicinsk biokemi och mikrobiologi
(creator_code:org_t)
Related titles
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In:EMBO Journal20, s. 864-8710261-41891460-2075
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