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Microsecond time-re...
Microsecond time-resolved X-ray scattering by utilizing MHz repetition rate at second-generation XFELs
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- Konold, Patrick (author)
- Uppsala universitet,Molekylär biofysik
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- Monrroy, Leonardo (author)
- Uppsala universitet,Biokemi
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- Bellisario, Alfredo (author)
- Uppsala universitet,Molekylär biofysik
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- de Matos Filipe, Diogo (author)
- Uppsala universitet,Molekylär biofysik
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Alvarez, Roberto (author)
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Bean, Richard (author)
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- Bielecki, Johan (author)
- Uppsala universitet,Molekylär biofysik
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Bódizs, Szabolcs (author)
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Ducroq, Gabriel (author)
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Grubmueller, Helmut (author)
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Kirian, Richard (author)
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Kloos, Marco (author)
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Koliyadu, Jayanat (author)
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Koua, Faisal (author)
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Larkiala, Taru (author)
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Letrun, Romain (author)
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Lindsten, Fredrik (author)
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Maihöfer, Michael (author)
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Martin, Andrew (author)
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Mészáros, Petra (author)
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Mutisya, Jennifer (author)
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Nimmrich, Amke (author)
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Okamoto, Kenta (author)
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Adam, Round (author)
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Sato, Tokushi (author)
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Valerio, Joana (author)
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- Westphal, Daniel (author)
- Uppsala universitet,Molekylär biofysik
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- Wollter, August (author)
- Uppsala universitet,Molekylär biofysik
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- Yenupuri, Tej Varma (author)
- Uppsala universitet,Molekylär biofysik
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- You, Tong (author)
- Uppsala universitet,Molekylär biofysik
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- Maia, Filipe (author)
- Uppsala universitet,Molekylär biofysik
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- Westenhoff, Sebastian (author)
- Uppsala universitet,Biokemi
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(creator_code:org_t)
- English.
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
Close
- Detecting microsecond structural perturbations in biomolecules has wide relevance inbiology, chemistry, and medicine. Here, we show how MHz repetition rates at X-ray freeelectron lasers (XFELs) can be used to produce microsecond time-series of proteinscattering with exceptionally low noise levels of 0.001%. We demonstrate the approach byderiving new mechanistic insight into Jɑ helix unfolding of a Light-Oxygen-Voltage (LOV)photosensory domain. This time-resolved acquisition strategy is easy to implement andwidely applicable for direct observation of structural dynamics of many biochemicalprocesses.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Keyword
- free-electron lasers
- time-resolved studies
- SAXS
- WAXS
- sample delivery
- XFELs
Publication and Content Type
- vet (subject category)
- ovr (subject category)
To the university's database
- By the author/editor
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Konold, Patrick
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Monrroy, Leonard ...
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Bellisario, Alfr ...
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de Matos Filipe, ...
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Alvarez, Roberto
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Bean, Richard
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show more...
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Bielecki, Johan
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Bódizs, Szabolcs
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Ducroq, Gabriel
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Grubmueller, Hel ...
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Kirian, Richard
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Kloos, Marco
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Koliyadu, Jayana ...
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Koua, Faisal
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Larkiala, Taru
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Letrun, Romain
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Lindsten, Fredri ...
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Maihöfer, Michae ...
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Martin, Andrew
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Mészáros, Petra
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Mutisya, Jennife ...
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Nimmrich, Amke
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Okamoto, Kenta
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Adam, Round
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Sato, Tokushi
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Valerio, Joana
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Westphal, Daniel
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Wollter, August
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Yenupuri, Tej Va ...
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You, Tong
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Maia, Filipe
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Westenhoff, Seba ...
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show less...
- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
- By the university
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Uppsala University