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Investigation of su...
Investigation of supramolecular structures in various aqueous solutions of an amyloid forming peptide using small-angle X-ray scattering
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- Brunzell, Ellen (author)
- Uppsala universitet,Institutionen för läkemedelskemi,Farmaceutisk fysikalisk kemi
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- Sigfridsson, Kalle (author)
- AstraZeneca, Adv Drug Delivery, Pharmaceut Sci, R&D, S-43183 Gothenburg, Sweden.
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- Gedda, Lars (author)
- Uppsala universitet,Fysikalisk kemi
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- Edwards, Katarina (author)
- Uppsala universitet,Fysikalisk kemi
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- Bergström, L. Magnus (author)
- Uppsala universitet,Institutionen för läkemedelskemi,Farmaceutisk fysikalisk kemi
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(creator_code:org_t)
- Royal Society of Chemistry, 2024
- 2024
- English.
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In: Soft Matter. - : Royal Society of Chemistry. - 1744-683X .- 1744-6848. ; 20:10, s. 2272-2279
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https://uu.diva-port... (primary) (Raw object)
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Abstract
Subject headings
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- Aggregation of peptide molecules into amyloid fibrils is a characteristic feature of several degenerative diseases. However, the details behind amyloid-formation, and other self-assembled peptide aggregates, remain poorly understood. In this study, we have used small-angle X-ray scattering (SAXS), static and dynamic light scattering (SLS and DLS) as well as cryogenic transmission electron microscopy (cryo-TEM) to determine the structural geometry of self-assembled peptide aggregates in various dilute aqueous solutions. Pramlintide was used as a model peptide to assess the aggregation behaviour of an amyloid-forming peptide. The effects of adding sodium chloride (NaCl), sodium thiocyanate (NaSCN), and sodium fluoride (NaF) and the co-solvent dimethyl sulfoxide (DMSO) on the aggregation behaviour were studied. Our scattering data analysis demonstrates that small oligomeric fibrils aggregate to form networks of supramolecular assemblies with fractal dimensions. The choice of anion in small amounts of added salt has a significant impact on the size of the fibrils as well as on the fractal dimensions of supramolecular clusters. In DMSO the fractal dimension decreased with increasing DMSO concentration, indicating the formation of a less compact structure of the supramolecular assemblies. The peptide pramlintide forms oligomeric species in solution, which make up a supramolecular network characterised by fractal dimensions. The fractal dimension of the network depends on solvent additive.
Subject headings
- NATURVETENSKAP -- Kemi -- Fysikalisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Physical Chemistry (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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