onr:"swepub:oai:DiVA.org:uu-52970"
Search: onr:"swepub:oai:DiVA.org:uu-52970" > Oscillatory Ca2+ si...
- 1 of 1
- Previous record
- Next record
- To hitlist
Oscillatory Ca2+ signaling in somatostatin-producing cells from the human pancreas
-
- Berts, Alf (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
- Liu, Yi-Jia (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
- Gylfe, Erik, 1947- (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
- show more...
- show less...
- (creator_code:org_t)
- 1997
- 1997
- English.
- In: Metabolism. - 0026-0495 .- 1532-8600. ; 46:4, s. 366-369
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Oscillatory Ca2+ signaling was studied in human somatostatin-releasing pancreatic δ cells identified by immunostaining. A ratiometric fura-2 technique was used for measuring cytoplasmic concentrations of Ca2+ and Sr2+ in δ cells exposed to the respective cation. Rhythmic activity in terms of slow (frequency, 0.1 to 0.4 per minute) oscillations from close to the basal level was seen in the presence of 3 to 20 mmol/L glucose during superfusion with medium containing 2.6 to 5 mmol/L Ca2+ or 5 mmol/L Sr2. These oscillations could be transformed into a sustained increase by decreasing extracellular Ca2+ or adding 1 mmol/L tolbutamide or 20 nmol/L glucagon. Addition of glucagon to a medium containing 20 mmol/L glucose resulted in the generation of short (< 30 seconds) transients, which disappeared upon exposure to 100 nmol/L of the intracellular Ca2+-adenosine triphosphatase (ATPase) inhibitor thapsigargin. When analyzing small aggregates of islet cells, it became evident that oscillatory activity in δ cells can be synchronous with that in adjacent non—δ cells. It is concluded that secretion of pancreatic somatostatin in man involves Ca2+ signaling similar to that regulating the pulsatile release of insulin.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Cell- och molekylärbiologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Cell and Molecular Biology (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Berts, Alf
- Liu, Yi-Jia
- Gylfe, Erik, 194 ...
- Hellman, Bo
- About the subject
-
- MEDICAL AND HEALTH SCIENCES
- MEDICAL AND HEAL ...
- and Basic Medicine
- and Cell and Molecul ...
- Articles in the publication
- Metabolism
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
