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High-throughput mea...
High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler
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- Sych, Taras (author)
- Karolinska Inst, Dept Womens & Childrens Hlth, Sci Life Lab, Solna, Sweden.
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- Schlegel, Jan (author)
- Karolinska Inst, Dept Womens & Childrens Hlth, Sci Life Lab, Solna, Sweden.
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- Barriga, Hanna M. G. (author)
- Karolinska Institutet,Karolinska Inst, Dept Med Biochem & Biophys, Stockholm, Sweden.
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- Ojansivu, Miina (author)
- Karolinska Institutet,Karolinska Inst, Dept Med Biochem & Biophys, Stockholm, Sweden.
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- Hanke, Leo (author)
- Karolinska Institutet,Karolinska Inst, Div Infect Dis, Dept Med Solna, Stockholm, Sweden.;Karolinska Inst, Ctr Mol Med, Stockholm, Sweden.
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- Weber, Florian (author)
- Karolinska Inst, Dept Womens & Childrens Hlth, Sci Life Lab, Solna, Sweden.;Univ Appl Sci Upper Austria, Dept Med Engn, Linz, Austria.
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- Bostancioglu, R. Beklem (author)
- Karolinska Inst, Dept Lab Med, Huddinge, Sweden.
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- Ezzat, Kariem (author)
- Karolinska Inst, Dept Lab Med, Huddinge, Sweden.
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- Stangl, Herbert (author)
- Med Univ Vienna, Inst Med Chem, Ctr Pathobiochem & Genet, Vienna, Austria.
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- Plochberger, Birgit (author)
- Univ Appl Sci Upper Austria, Dept Med Engn, Linz, Austria.;LBG Ludwig Boltzmann Inst Traumatol, Nanoscopy, Vienna, Austria.
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- Laurencikiene, Jurga (author)
- Karolinska Institutet,Karolinska Inst, Dept Med Huddinge, Lipid Lab, Stockholm, Sweden.
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- El Andaloussi, Samir (author)
- Karolinska Institutet,Karolinska Inst, Dept Lab Med, Huddinge, Sweden.
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- Fuerth, Daniel (author)
- Uppsala universitet,Institutionen för immunologi, genetik och patologi,Science for Life Laboratory, SciLifeLab
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- Stevens, Molly M. (author)
- Karolinska Institutet,Karolinska Inst, Dept Med Biochem & Biophys, Stockholm, Sweden.;Imperial Coll London, Dept Mat, Dept Bioengn, London, England.;Imperial Coll London, Inst Biomed Engn, London, England.
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- Sezgin, Erdinc (author)
- Karolinska Institutet,Karolinska Inst, Dept Womens & Childrens Hlth, Sci Life Lab, Solna, Sweden.
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Karolinska Institutet Karolinska Inst, Dept Womens & Childrens Hlth, Sci Life Lab, Solna, Sweden (creator_code:org_t)
- Nature Publishing Group, 2024
- 2024
- English.
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In: Nature Biotechnology. - : Nature Publishing Group. - 1087-0156 .- 1546-1696. ; 42:4
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https://uu.diva-port... (primary) (Raw object)
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Abstract
Subject headings
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- Lipid nanoparticles, viruses, exosomes and liposomes are characterized by analysis of fluorescence fluctuations. We introduce a method, single-particle profiler, that provides single-particle information on the content and biophysical properties of thousands of particles in the size range 5-200 nm. We use our single-particle profiler to measure the messenger RNA encapsulation efficiency of lipid nanoparticles, the viral binding efficiencies of different nanobodies, and the biophysical heterogeneity of liposomes, lipoproteins, exosomes and viruses.
Subject headings
- NATURVETENSKAP -- Biologi -- Biofysik (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biophysics (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Sych, Taras
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Schlegel, Jan
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Barriga, Hanna M ...
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Ojansivu, Miina
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Hanke, Leo
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Weber, Florian
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show more...
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Bostancioglu, R. ...
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Ezzat, Kariem
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Stangl, Herbert
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Plochberger, Bir ...
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Laurencikiene, J ...
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El Andaloussi, S ...
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Fuerth, Daniel
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Stevens, Molly M ...
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Sezgin, Erdinc
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biophysics
- Articles in the publication
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Nature Biotechno ...
- By the university
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Uppsala University
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Karolinska Institutet