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Molecular insights to in vitro biocompatibility of endodontic Pulpotec with macrophages determined by oxidative stress and apoptosis
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- Mohanty, Ankita (author)
- KIIT DU, KIIT Sch Dent Sci, Bhubaneswar 751024, Orissa, India.
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- Patro, Swadheena (author)
- KIIT DU, KIIT Sch Dent Sci, Bhubaneswar 751024, Orissa, India.
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- Jha, Ealisha (author)
- KIIT DU, Sch Biotechnol, Bhubaneswar 751024, Orissa, India.
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- Patel, Paritosh (author)
- KIIT DU, Sch Biotechnol, Bhubaneswar 751024, Orissa, India.;Kwangwoon Univ, Plasma Biosci Res Ctr, Dept Elect & Biol Phys, Seoul 01897, South Korea.
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- Nandi, Aditya (author)
- KIIT DU, Sch Biotechnol, Bhubaneswar 751024, Orissa, India.
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- Sinha, Adrija (author)
- KIIT DU, Sch Biotechnol, Bhubaneswar 751024, Orissa, India.
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- Naser, Shaikh Sheeran (author)
- KIIT DU, Sch Biotechnol, Bhubaneswar 751024, Orissa, India.
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- Das, Antarikshya (author)
- KIIT DU, KIIT Sch Dent Sci, Bhubaneswar 751024, Orissa, India.
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- Panda, Pritam Kumar, PhD Student, 1991- (author)
- Uppsala universitet,Materialteori,Condensed Matter Theory Grp
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- Rout, Prabhat Kumar (author)
- KIIT DU, Sch Biotechnol, Bhubaneswar 751024, Orissa, India.
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- Mishra, Richa (author)
- Parul Univ, Vadodara 391760, Gujarat, India.
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- Kaushik, Nagendra Kumar (author)
- Kwangwoon Univ, Plasma Biosci Res Ctr, Dept Elect & Biol Phys, Seoul 01897, South Korea.
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- Singh, Deobrat (author)
- Uppsala universitet,Materialteori,Condensed Matter Theory Grp
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- Suar, Mrutyunjay (author)
- KIIT DU, Sch Biotechnol, Bhubaneswar 751024, Orissa, India.
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- Verma, Suresh K. (author)
- KIIT DU, Sch Biotechnol, Bhubaneswar 751024, Orissa, India.
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KIIT DU, KIIT Sch Dent Sci, Bhubaneswar 751024, Orissa, India KIIT DU, Sch Biotechnol, Bhubaneswar 751024, Orissa, India. (creator_code:org_t)
- Elsevier, 2024
- 2024
- English.
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In: Biomedicine and Pharmacotherapy. - : Elsevier. - 0753-3322 .- 1950-6007. ; 176
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Abstract
Subject headings
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- Pulp therapy has been emerged as a one of the efficient therapies in the field of endodontics. Among different types of new endodontic materials, pulpotec has been materialized as a recognized material for vital pulp therapy. However, its efficacy has been challenged due to lack of information about its cellular biocompatibility. This study evaluates the mechanistic biocompatibility of pulpotec cement with macrophage cells (RAW 264.7) at cellular and molecular level. The biocompatibility was evaluated using experimental and computational techniques like MTT assay, oxidative stress analysis and apoptosis analysis through flow cytometry and fluorescent microscopy. The results showed concentration-dependent cytotoxicity of pulpotec cement extract to RAW 264.7 cells with an LC 50 of X/10 -X/20. The computational analysis depicted the molecular interaction of pulpotec cement extract components with metabolic proteins like Sod1 and p53. The study revealed the effects of Pulpotec cement's extract, showing a concentration-dependent induction of oxidative stress and apoptosis. These effects were due to influential structural and functional abnormalities in the Sod1 and p53 proteins, caused by their molecular interaction with internalized components of Pulpotec cement. The study provided a detailed view on the utility of Pulpotec in endodontic applications, highlighting its biomedical aspects.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Odontologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dentistry (hsv//eng)
Keyword
- Pulpotec
- Oxidative stress
- Apoptosis
- Macrophages
- Biocompatibility
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Mohanty, Ankita
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Patro, Swadheena
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Jha, Ealisha
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Patel, Paritosh
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Nandi, Aditya
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Sinha, Adrija
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show more...
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Naser, Shaikh Sh ...
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Das, Antarikshya
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Panda, Pritam Ku ...
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Rout, Prabhat Ku ...
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Mishra, Richa
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Kaushik, Nagendr ...
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Singh, Deobrat
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Suar, Mrutyunjay
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Verma, Suresh K.
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show less...
- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Dentistry
- Articles in the publication
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Biomedicine and ...
- By the university
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Uppsala University