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- Orlova, AnnaUppsala universitet,Enheten för biomedicinsk strålningsvetenskap (author)
Cellular processing of 125I- and 111in-labeled epidermal growth factor (EGF) bound to cultured A431 tumor cells
- Article/chapterEnglish2000
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- 2000
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- LIBRIS-ID:oai:DiVA.org:uu-61313
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-61313URI
- https://doi.org/10.1016/S0969-8051(00)00148-7DOI
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- Language:English
- Summary in:English
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- Low molecular weight of epidermal growth factor (EGF) enables better intratumoral penetration in comparison with larger targeting proteins, but the cellular retention of EGF-associated radioactivity is poor for directly iodinated EGF. An attempt was made to improve intracellular retention by the use of metal-diethylenetriaminepentaacetic acid or nonphenolic linker (N-succinimidyl-para-iodobenzoate) as labeling agents. The use of nonphenolic linker did not improve retention of the radioactivity in A431 carcinoma cell line. The use of the radiometal label provided an appreciable prolongation of radioactivity residence inside the cell.
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- Bruskin, Alexander (author)
- Sjöström, AnnaUppsala universitet,Enheten för biomedicinsk strålningsvetenskap(Swepub:uu)annsjost (author)
- Lundqvist, HansUppsala universitet,Enheten för biomedicinsk strålningsvetenskap(Swepub:uu)hanslund (author)
- Gedda, LarsUppsala universitet,Enheten för biomedicinsk strålningsvetenskap(Swepub:uu)larsgedd (author)
- Tolmavhev, VladimirUppsala universitet,Enheten för biomedicinsk strålningsvetenskap(Swepub:uu)vladtolm (author)
- Uppsala universitetEnheten för biomedicinsk strålningsvetenskap (creator_code:org_t)
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- In:Nuclear Medicine and Biology27:8, s. 827-8350969-80511872-9614
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