Search: onr:"swepub:oai:DiVA.org:uu-65540" >
Effects of interfer...
Effects of interferon alpha on the expression of p21cip1/waf1 and cellcycle distribution in carcinoid tumors.
-
- Zhou, Y (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
- Wang, S (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
Yue, BG (author)
-
show more...
-
- Gobl, A (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
- Oberg, K (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
-
show less...
-
(creator_code:org_t)
- 2002
- 2002
- English.
-
In: Cancer Invest. ; 20, s. 348-
- Related links:
-
https://urn.kb.se/re...
Abstract
Subject headings
Close
- Interferon alpha (IFN-alpha) has been shown to produce antitumor effects in 50-80% of carcinoid tumor patients and has demonstrated anti-proliferative effects in carcinoid tumor cells, but the mechanism is not well established. This study presents evidence that in a carcinoid tumor cell line, Bon1, IFN-alpha increases the expression of p21 and promotes nuclear translocation of endogenous p21. Furthermore, immunoprecipitation experiments demonstrated that p21 formed immuno-complexes with Stat1 and Stat2 in the nucleus of cells. Interferon alpha can decrease G1- and G2-phase cells, but increase S-phase population. The p21 mRNA expression is inversely correlated to the G1 population (r = -0.933, P < 0.05) and positively correlated to the S-phase population (r = 0.901, P < 0.05). In addition, IFN-alpha inhibited cyclin dependent kinases (CDK), CDK2-, CDK3-, CDK4-, and cyclin E- but not cyclin A-associated kinase activities. Immunodepletion of p21 resulted in a significant enhancement of CDK3 kinase activity (approximately 1.6-fold increase). These results suggest that the mechanism of antitumor and cell cycle regulation of IFN-alpha in carcinoid tumors may, at least in part, be p21-dependent. Based on these results, we conclude that IFN-alpha exerts antitumor effects by increased p21 expression in neuroendocrine tumors.
Keyword
- interferon alpha
- gene expression
- p21Cip1/Waf1
- signal transduction
- carcinoid
- neuroendocrine tumor
- cell cycle
- cyclin dependent kinase
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database