Precursor of human adenovirus core polypeptide Mu targets the nucleolus and modulates the expression of E2 proteins

We have examined the subcellular localization properties of human adenovirus 2 (HAdV-2) preMu and mature Mu (pX) proteins as fusions with enhanced green fluorescence protein (EGFP). We determined that preMu is exclusively a nucleolar protein with a single nucleolar accumulation signal within the Mu sequence. In addition, we noted that both preMu-EGFP and Mu-EGFP are excluded from adenovirus DNA-binding protein (DBP)-rich replication centres in adenovirus-infected cells. Surprisingly, we observed that cells in which preMu-EGFP (but not Mu-EGFP) is transiently expressed prior to or shortly after infection with Ad2 did not express late adenovirus genes. Further investigation suggested this might be due to a failure to express pre-terminal protein (preTP) from the E2 region, despite expression of another E2 protein, DBP. Deletion mutagenesis identified a highly conserved region in the C terminus of preMu responsible for these observations. Thus our data suggest that preMu may play a role in modulating accumulation of proteins from the E2 region.

Lawrence, F J (author)
Dauksaite, VUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi (author)
Akusjärvi, GöranUppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi(Swepub:uu)gak30132 (author)
Blair, G E (author)
Matthews, D A (author)
Uppsala universitetInstitutionen för medicinsk biokemi och mikrobiologi (creator_code:org_t)

In:Journal of General Virology: Microbiology Society85:Pt 1, s. 185-1960022-13171465-2099

By the author/editor: Lee, T W R; Lawrence, F J; Dauksaite, V; Akusjärvi, Göran; Blair, G E; Matthews, D A

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