An optimistic view for quantifying mRNA in post-mortem human brain

Quantitative human mRNA data are derived from post-mortem or biopsied tissue. RNA degradation, poor replication, a large mRNA variance and confounding factors such as brain pH and age of death are often cited, however, as objections to the data's reliability. A central question is whether post-mortem human mRNA can be treated as a statistically ordered system. TaqMan real-time RT-PCR was used to measure seven mRNAs in 513 cortical samples taken from 90 Alzheimer's disease and 81 control brains. Despite a high mRNA variance strong correlations were found between the mRNA transcripts in a single brain. Where a brain has a high/low level of one mRNA, the same brain invariably has a high/low level of other mRNAs; correlated order is present and allows removal of that source of variation common to all genes. Although levels of mRNA are highly variable between subjects (>1000-fold), quantitative order is present in post-mortem human mRNA, allowing effects due to pathology or gender to be isolated and tested for significance.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Neurovetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Neurosciences hsv//eng
NATURVETENSKAP Biologi Genetik hsv//swe
NATURAL SCIENCES Biological Sciences Genetics hsv//eng
Aged
Aged; 80 and over
Alzheimer Disease/metabolism
Amyloid beta-Protein Precursor/genetics
Brain Chemistry
Cadaver
Case-Control Studies
Comparative Study
Female
Glial Fibrillary Acidic Protein/genetics
Glyceraldehyde-3-Phosphate Dehydrogenases/genetics
Humans
Linear Models
Male
Microtubule-Associated Proteins/genetics
Middle Aged
Nerve Tissue Proteins/genetics
Peptide Fragments/genetics
Postmortem Changes
RNA Stability
RNA; Messenger/*analysis/chemistry
Reproducibility of Results
Reverse Transcriptase Polymerase Chain Reaction/methods
Sex Factors
Taq Polymerase
Neurobiology
Neurobiologi
Genetics
Genetik

Virley, David J (author)
Costandi, Moheb (author)
Coombes, Robert (author)
Moss, Stephen J (author)
Mudge, Anne W (author)
Jazin, ElenaUppsala universitet,Evolutionsbiologi,Behavioral Genetics(Swepub:uu)elenjazi (author)
Cairns, Nigel J (author)
Uppsala universitetEvolutionsbiologi (creator_code:org_t)

By the author/editor: Preece, Paul; Virley, David J; Costandi, Moheb; Coombes, Robert; Moss, Stephen J; Mudge, Anne W; show more...; Jazin, Elena; Cairns, Nigel J; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Neurosciences

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