Search: onr:"swepub:oai:DiVA.org:uu-72472" >
An optimistic view ...
An optimistic view for quantifying mRNA in post-mortem human brain
- Article/chapterEnglish2003
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-72472
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-72472URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Quantitative human mRNA data are derived from post-mortem or biopsied tissue. RNA degradation, poor replication, a large mRNA variance and confounding factors such as brain pH and age of death are often cited, however, as objections to the data's reliability. A central question is whether post-mortem human mRNA can be treated as a statistically ordered system. TaqMan real-time RT-PCR was used to measure seven mRNAs in 513 cortical samples taken from 90 Alzheimer's disease and 81 control brains. Despite a high mRNA variance strong correlations were found between the mRNA transcripts in a single brain. Where a brain has a high/low level of one mRNA, the same brain invariably has a high/low level of other mRNAs; correlated order is present and allows removal of that source of variation common to all genes. Although levels of mRNA are highly variable between subjects (>1000-fold), quantitative order is present in post-mortem human mRNA, allowing effects due to pathology or gender to be isolated and tested for significance.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Virley, David J
(author)
-
Costandi, Moheb
(author)
-
Coombes, Robert
(author)
-
Moss, Stephen J
(author)
-
Mudge, Anne W
(author)
-
Jazin, ElenaUppsala universitet,Evolutionsbiologi,Behavioral Genetics(Swepub:uu)elenjazi
(author)
-
Cairns, Nigel J
(author)
-
Uppsala universitetEvolutionsbiologi
(creator_code:org_t)
Related titles
-
In:Brain Research. Molecular Brain Research116:1-2, s. 7-160169-328X1872-6941
Internet link
Find in a library
To the university's database