onr:"swepub:oai:DiVA.org:uu-72965"
Search: onr:"swepub:oai:DiVA.org:uu-72965" > Proteomics-based id...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Grimsby, SusanneUppsala universitet,Ludwiginstitutet för cancerforskning (author)
Proteomics-based identification of proteins interacting with Smad3 : SREBP-2 forms a complex with Smad3 and inhibits its transcriptional activity
- Article/chapterEnglish2004
Publisher, publication year, extent ...
- 2004-10-07
- Wiley,2004
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-72965
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-72965URI
- https://doi.org/10.1016/j.febslet.2004.09.069DOI
- http://kipublications.ki.se/Default.aspx?queryparsed=id:110654603URI
Supplementary language notes
- Language:English
- Summary in:English &language:-1_t
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Smad3 is an important component of transforming growth factor-beta (TGFbeta) intracellular signalling. To identify novel interacting proteins of Smad3, we performed pull-down assays with Smad3 constructs fused to glutathione-S-transferase. Proteins which formed complexes with these constructs were analyzed by two-dimensional gel electrophoresis, and were identified by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. We identified 14 proteins interacting with the Smad3 construct lacking the N-terminal Mad homology domain 1 (MH1), and 12 proteins interacting with the construct lacking the C-terminal MH2 domain. Proteins involved in signalling processes, in metabolism regulation, novel proteins, and components of cytoskeleton form four groups of interacting proteins. Interactions of AGP7, sex-determining region Y protein, actin beta and sterol regulatory element binding protein-2 (SREBP-2) proteins with Smad3 constructs were confirmed by immunoblotting with specific antibodies. Interaction of Smad3 with SREBP-2 was also confirmed by co-immunoprecipitation of myc-Smad3 and Flag-SREBP-2 upon expression in mammalian cells. We found that SREBP-2 inhibited the transcriptional activity of Smad3 in luciferase reporter assays.
Subject headings and genre
- Animals
- Cell Line
- DNA-Binding Proteins/*metabolism
- Electrophoresis; Gel; Two-Dimensional
- Humans
- Protein Binding
- Proteome
- Research Support; Non-U.S. Gov't
- Spectrometry; Mass; Matrix-Assisted Laser Desorption-Ionization
- Trans-Activators/*metabolism
- Transcription Factors/*metabolism
- Transcription; Genetic
Added entries (persons, corporate bodies, meetings, titles ...)
- Jaensson, HannaUppsala universitet,Ludwiginstitutet för cancerforskning (author)
- Dubrovska, AnnaUppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)andub498 (author)
- Lomnytska, MartaUppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)marlo829 (author)
- Hellman, UlfUppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)ulfhm (author)
- Souchelnytskyi, SerhiyKarolinska Institutet,Uppsala universitet,Ludwiginstitutet för cancerforskning(Swepub:uu)sergsouc (author)
- Uppsala universitetLudwiginstitutet för cancerforskning (creator_code:org_t)
Related titles
- In:FEBS Letters: Wiley577:1-2, s. 93-1000014-57931873-3468
Internet link
- https://doi.org/10.1016/j.febslet.2004.09.069Fulltext
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed&cmd=Retrieve&list_uids=15527767&dopt=Citation
- https://febs.onlinelibrary.wiley.com/doi/pdfdirect/10.1016/j.febslet.2004.09.069
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-72965
- https://doi.org/10.1016/j.febslet.2004.09.069
- http://kipublications.ki.se/Default.aspx?queryparsed=id:110654603
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Grimsby, Susanne
- Jaensson, Hanna
- Dubrovska, Anna
- Lomnytska, Marta
- Hellman, Ulf
- Souchelnytskyi, ...
- Articles in the publication
- FEBS Letters
- By the university
- Uppsala University
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
