Search: onr:"swepub:oai:DiVA.org:uu-73053" >
Effect of polyethyl...
-
Edwards, KatarinaUppsala universitet,Fysikalisk kemi I
(author)
Effect of polyethyleneglycol-phospholipids on aggregate structure in preparations of small unilamellar liposomes
- Article/chapterEnglish1997
Publisher, publication year, extent ...
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-73053
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-73053URI
-
https://doi.org/10.1016/S0006-3495(97)78066-4DOI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:vet swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
Phospholipids with covalently attached poly(ethylene glycol) (PEG lipids) are commonly used for the preparation of long circulating liposomes. Although it is well known that lipid/PEG-lipid mixed micelles may form above a certain critical concentration of PEG-lipid, little is known about the effects of PEG-lipids on liposome structure and leakage at submicellar concentrations. In this study we have used cryogenic transmission electron microscopy to investigate the effect of PEG(2000)-PE on aggregate structure in preparations of liposomes with different membrane compositions. The results reveal a number of important aggregate structures not documented before. The micrographs show that enclosure of PEG-PE induces the formation of open bilayer discs at concentrations well below those where mixed micelles begin to form. The maximum concentration of PEG-lipid that may be incorporated without alteration of the liposome structure depends on the phospholipid chain length, whereas phospholipid saturation or the presence of cholesterol has little or no effect. The presence of cholesterol does, however, affect the shape of the mixed micelles formed at high concentrations of PEG-lipid. Threadlike micelles form in the absence of cholesterol but adapt a globular shape when cholesterol is present.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
-
Johnsson, MUppsala universitet,Fysikalisk kemi I
(author)
-
Karlsson, GUppsala universitet,Fysikalisk kemi I
(author)
-
Silvander, MUppsala universitet,Fysikalisk kemi I
(author)
-
Uppsala universitetFysikalisk kemi I
(creator_code:org_t)
Related titles
-
In:Biophysical Journal73:1, s. 258-2660006-34951542-0086
Internet link
Find in a library
To the university's database