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Effects of 2-methox...
Effects of 2-methoxyestradiol on proliferation, apoptosis and PET-tracer uptake in human prostate cancer cell aggregates
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- Davoodpour, Padideh (author)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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Bergström, Mats (author)
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- Landström, Maréne (author)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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(creator_code:org_t)
- Elsevier BV, 2004
- 2004
- English.
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In: Nuclear Medicine and Biology. - : Elsevier BV. - 0969-8051 .- 1872-9614. ; 31:7, s. 867-874
- Related links:
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http://www.ncbi.nlm....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The purpose of this study was to investigate the potential use of PET in vivo to record cytotoxic effects of 2-methoxyestradiol (2-ME), an endogenous metabolite of 17beta-estradiol. The anti-proliferative and pro-apoptotic effects of 2-ME on human prostate cancer cell (PC3) aggregates in vitro, were correlated with the uptake of fluoro-deoxy-D-glucose, FMAU and choline labelled with 18F, 11C, or 3H. 2-ME clearly reduced growth of PC3 aggregates and induced apoptosis in a dose-dependent manner. However, the uptake of the putative proliferation markers 11C-FMAU or 3H-choline failed to record the growth inhibitory effects of 2-ME on PC3 cell aggregates. The uptake of 18F-FDG was used as a marker for effects on cellular metabolism and also failed to show any dose-dependent effects in PC3 aggregates. The use of these PET-tracers in vivo is therefore not recommended in order to evaluate the cytotoxic effects of 2-ME on human prostate cancer cells.
Keyword
- Antineoplastic Agents/administration & dosage
- Apoptosis/*drug effects
- Cell Aggregation/drug effects
- Cell Line; Tumor
- Cell Proliferation/*drug effects
- Comparative Study
- Dose-Response Relationship; Drug
- Estradiol/*administration & dosage/*analogs & derivatives
- Humans
- Male
- Neoplasm Staging/methods
- Positron-Emission Tomography/*methods
- Prostatic Neoplasms/drug therapy/*metabolism/pathology/*radionuclide imaging
- Radioisotopes/*diagnostic use/*pharmacokinetics
- Radiopharmaceuticals/diagnostic use/pharmacokinetics
- Reproducibility of Results
- Research Support; Non-U.S. Gov't
- Sensitivity and Specificity
Publication and Content Type
- ref (subject category)
- art (subject category)
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