Effects of 2-methoxyestradiol on proliferation, apoptosis and PET-tracer uptake in human prostate cancer cell aggregates

Davoodpour, Padideh (author): Uppsala universitet,Ludwiginstitutet för cancerforskning

Bergström, Mats (author)

Landström, Maréne (author): Uppsala universitet,Ludwiginstitutet för cancerforskning

(creator_code:org_t)

Elsevier BV, 2004
2004
English.
In: Nuclear Medicine and Biology. - : Elsevier BV. - 0969-8051 .- 1872-9614. ; 31:7, s. 867-874

Related links:: http://www.ncbi.nlm....; show more...; https://urn.kb.se/re...; https://doi.org/10.1...; show less...

Abstract Subject headings

The purpose of this study was to investigate the potential use of PET in vivo to record cytotoxic effects of 2-methoxyestradiol (2-ME), an endogenous metabolite of 17beta-estradiol. The anti-proliferative and pro-apoptotic effects of 2-ME on human prostate cancer cell (PC3) aggregates in vitro, were correlated with the uptake of fluoro-deoxy-D-glucose, FMAU and choline labelled with 18F, 11C, or 3H. 2-ME clearly reduced growth of PC3 aggregates and induced apoptosis in a dose-dependent manner. However, the uptake of the putative proliferation markers 11C-FMAU or 3H-choline failed to record the growth inhibitory effects of 2-ME on PC3 cell aggregates. The uptake of 18F-FDG was used as a marker for effects on cellular metabolism and also failed to show any dose-dependent effects in PC3 aggregates. The use of these PET-tracers in vivo is therefore not recommended in order to evaluate the cytotoxic effects of 2-ME on human prostate cancer cells.

Antineoplastic Agents/administration & dosage
Apoptosis/*drug effects
Cell Aggregation/drug effects
Cell Line; Tumor
Cell Proliferation/*drug effects
Comparative Study
Dose-Response Relationship; Drug
Estradiol/*administration & dosage/*analogs & derivatives
Humans
Male
Neoplasm Staging/methods
Positron-Emission Tomography/*methods
Prostatic Neoplasms/drug therapy/*metabolism/pathology/*radionuclide imaging
Radioisotopes/*diagnostic use/*pharmacokinetics
Radiopharmaceuticals/diagnostic use/pharmacokinetics
Reproducibility of Results
Research Support; Non-U.S. Gov't
Sensitivity and Specificity

By the author/editor: Davoodpour, Padi ...; Bergström, Mats; Landström, Marén ...

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