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Induction of apopto...
Induction of apoptosis and G2/M arrest by 2-methoxyestradiol in human cervical cancer HeLaS3 cells.
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- Li, Li (author)
- Uppsala universitet,Institutionen för kvinnors och barns hälsa,Klinisk och experimentell reproduktionsbiologi/Olovsson
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- Bu, Shizhong (author)
- Ludwiginstitutet för Cancerforskning
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- Bäckström, Torbjörn (author)
- Umeå universitet,Obstetrik och gynekologi
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- Landström, Maréne (author)
- Ludwiginstitutet för Cancerforskning
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Ulmsten, Ulf (author)
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- Fu, Xin (author)
- Uppsala universitet,Institutionen för kvinnors och barns hälsa,Klinisk och experimentell reproduktionsbiologi/Olovsson
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(creator_code:org_t)
- Athens : International institute for anticancer research, 2004
- 2004
- English.
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In: Anticancer Res. - Athens : International institute for anticancer research. - 0250-7005 .- 1791-7530. ; 24:2B, s. 873-80
- Related links:
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http://www.ncbi.nlm....
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https://urn.kb.se/re...
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Abstract
Subject headings
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- BACKGROUND: It has been demonstrated that 2-Methoxyestradiol (2-ME), one of the estrogen metabolites, induces apoptosis in many different tumor cell lines. In the present study, the effects of 2-ME on human cervical cancer HeLaS3 cells and on normal cervical epithelial cells were evaluated. MATERIALS AND METHODS: Acridine orange staining, DNA fragmentation arrays and flow cytometry were used to measure the apoptosis and cell cycle progression. In addition, the effect of 2-ME on expression of iNOS was measured by Western blot. RESULTS: 2-ME inhibited the growth of HeLaS3 cells. This growth inhibition was accompanied by apoptosis and G2/M cell cycle arrest. 2-ME increased the expression of iNOS in parallel with apoptosis. Moreover, apoptosis was prevented by the iNOS inhibitor 1400W. 2-ME treatment resulted in a slight increase of the G2/M population, but no apoptosis, in normal cervical epithelial cells. There was no synergetic effect between E2 and 2-ME. CONCLUSION: 2-ME induced apoptosis via the iNOS pathway and caused G2/M cell cycle arrest in human cervical cancer HeLaS3 cells, but showed only slight effects on normal cervical epithelial cells. These data suggest that 2-ME might be an adjuvant agent in the treatment of cervical cancer.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Cancer och onkologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cancer and Oncology (hsv//eng)
Keyword
- Adenocarcinoma/*drug therapy/pathology
- Apoptosis/*drug effects
- Cell Cycle/*drug effects
- Cell Division/drug effects
- Cervix Neoplasms/*drug therapy/pathology
- Cervix Uteri/cytology/drug effects
- Epithelial Cells/cytology/drug effects
- Estradiol/*analogs & derivatives/*pharmacology
- Female
- G2 Phase/drug effects
- Hela Cells
- Humans
- Mitosis/drug effects
- Research Support; Non-U.S. Gov't
Publication and Content Type
- ref (subject category)
- art (subject category)
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