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Defective oligodend...
Defective oligodendrocyte development and severe hypomyelination in PDGF-A knockout mice.
- Article/chapterEnglish1999
Publisher, publication year, extent ...
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-73157
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-73157URI
Supplementary language notes
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Language:English
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Summary in:English
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Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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There is a class of oligodendrocyte progenitors, called O-2A progenitors, that is characterized by expression of platelet-derived growth factor alpha-receptors (PDGFRalpha). It is not known whether all oligodendrocytes are derived from these PDGFRalpha-progenitors or whether a subset(s) of oligodendrocytes develops from a different, PDGFR alpha-negative lineage(s). We investigated the relationship between PDGF and oligodendrogenesis by examining mice that lack either PDGF-A or PDGF-B. PDGF-A null mice had many fewer PDGFR alpha-progenitors than either wild-type or PDGF-B null mice, demonstrating that proliferation of these cells relies heavily (though not exclusively) on PDGF-AA homodimers. PDGF-A-deficient mice also had reduced numbers of oligodendrocytes and a dysmyelinating phenotype (tremor). Not all parts of the central nervous system (CNS) were equally affected in the knockout. For example, there were profound reductions in the numbers of PDGFR alpha-progenitors and oligodendrocytes in the spinal cord and cerebellum, but less severe reductions of both cell types in the medulla. This correlation suggests a close link between PDGFRalpha-progenitors and oligodendrogenesis in most or all parts of the CNS. We also provide evidence that myelin proteolipid protein (PLP/DM-20)-positive cells in the late embryonic brainstem are non-dividing cells, presumably immature oligodendrocytes, and not proliferating precursors.
Subject headings and genre
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Animals
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Brain/embryology
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Cell Differentiation
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Cell Division
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Central Nervous System/*embryology
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Mice
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Mice; Knockout
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Myelin Proteolipid Protein/physiology
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Myelin Sheath/*physiology
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Oligodendroglia/*physiology
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Platelet-Derived Growth Factor/genetics/*physiology
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Proto-Oncogene Proteins/genetics/physiology
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Proto-Oncogene Proteins c-sis
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Receptor; Platelet-Derived Growth Factor alpha
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Receptors; Platelet-Derived Growth Factor/genetics/physiology
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Research Support; Non-U.S. Gov't
Added entries (persons, corporate bodies, meetings, titles ...)
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Karlsson, L
(author)
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Hall, A C
(author)
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Abramsson, A
(author)
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Calver, A R
(author)
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Boström, HansUppsala universitet,Institutionen för genetik och patologi(Swepub:uu)habos227
(author)
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Willetts, K
(author)
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Bertold, C H
(author)
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Heath, J K
(author)
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Betsholtz, C
(author)
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Richardson, W D
(author)
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Uppsala universitetInstitutionen för genetik och patologi
(creator_code:org_t)
Related titles
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In:Development126:3, s. 457-670950-1991
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To the university's database
- By the author/editor
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Fruttiger, M
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Karlsson, L
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Hall, A C
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Abramsson, A
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Calver, A R
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Boström, Hans
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show more...
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Willetts, K
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Bertold, C H
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Heath, J K
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Betsholtz, C
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Richardson, W D
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show less...
- Articles in the publication
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Development
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Uppsala University