A microarray minisequencing system for pharmacogenetic profiling of antihypertensive drug response

Liljedahl, Ulrika (author): Uppsala universitet,Institutionen för medicinska vetenskaper,Department of Medical Sciences, Uppsala University, Uppsala, Sweden

Karlsson, Julia (author): Uppsala universitet,Institutionen för medicinska vetenskaper,Osteoporos,Department of Medical Sciences, Uppsala University, Uppsala, Sweden

Melhus, Håkan (author): Uppsala universitet,Institutionen för medicinska vetenskaper,Osteoporos,Department of Medical Sciences, Uppsala University, Uppsala, Sweden

Kurland, Lisa, 1960- (author): Uppsala universitet,Institutionen för medicinska vetenskaper,Akut- och internmedicin,Department of Medical Sciences, Uppsala University, Uppsala, Sweden

Lindersson, Marie (author): Department of Medical Sciences, Uppsala University, Uppsala, Sweden

Nyström, Fredrik, 1963- (author): Östergötlands Läns Landsting,Linköpings universitet,Hälsouniversitetet,Internmedicin,EMT-endo

Lind, Lars (author): Uppsala universitet,Institutionen för medicinska vetenskaper,Akut- och internmedicin,Department of Medical Sciences, Uppsala University, Uppsala, Sweden; Astra Zeneca R&D, Mölndal, Sweden

Syvänen, Ann-Christine (author): Uppsala universitet,Institutionen för medicinska vetenskaper,Department of Medical Sciences, Uppsala University, Uppsala, Sweden

(creator_code:org_t)

Ovid Technologies (Wolters Kluwer Health), 2003
2003
English.
In: Pharmacogenetics. - : Ovid Technologies (Wolters Kluwer Health). - 0960-314X .- 1473-561X. ; 13:1, s. 7-17

Related links:: http://www.ncbi.nlm....; show more...; https://urn.kb.se/re...; https://urn.kb.se/re...; https://doi.org/10.1...; http://kipublication...; https://urn.kb.se/re...; show less...

Abstract Subject headings

We aimed to develop a microarray genotyping system for multiplex analysis of a panel of single nucleotide polymorphisms (SNPs) in genes encoding proteins involved in blood pressure regulation, and to apply this system in a pilot study demonstrating its feasibility in the pharmacogenetics of hypertension. A panel of 74 SNPs in 25 genes involved in blood pressure regulation was selected from the SNP databases, and genotyped in DNA samples of 97 hypertensive patients. The patients had been randomized to double-blind treatment with either the angiotensin II type 1 receptor blocker irbesartan or the beta 1-adrenergic receptor blocker atenolol. Genotyping was performed using a microarray based DNA polymerase assisted 'minisequencing' single nucleotide primer extension assay with fluorescence detection. The observed genotypes were related to the blood pressure reduction using stepwise multiple regression analysis. The allele frequencies of the selected SNPs were determined in the Swedish population. The established microarray-based genotyping system was validated and allowed unequivocal multiplex genotyping of the panel of 74 SNPs in every patient. Almost 7200 SNP genotypes were generated in the study. Profiles of four or five SNP-genotypes that may be useful as predictors of blood pressure reduction after antihypertensive treatment were identified. Our results highlight the potential of microarray-based technology for SNP genotyping in pharmacogenetics.

MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Medicinsk genetik (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Medical Genetics (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Kardiologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Cardiac and Cardiovascular Systems (hsv//eng)

By the author/editor: Liljedahl, Ulrik ...; Karlsson, Julia; Melhus, Håkan; Kurland, Lisa, 1 ...; Lindersson, Mari ...; Kahan, Thomas; show more...; Nyström, Fredrik ...; Lind, Lars; Syvänen, Ann-Chr ...; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Medical Genetics

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Pharmacology and ...

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Cardiac and Card ...

By the university: Uppsala University; Örebro University; Karolinska Institutet; Linköping University

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