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Microdialysis-evalu...
Microdialysis-evaluated myocardial cyclooxygenase-mediated inflammation and early circulatory depression in porcine endotoxemia
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- Mutschler, Diana K. (author)
- Uppsala universitet,Institutionen för kirurgiska vetenskaper,Anaesthesiology and Intensive Care
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Eriksson, M. B. (author)
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Wikström, B. G. (author)
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- Lind, Lars (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Akut- och internmedicin
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- Larsson, Anders (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper
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- Berggren-Kiiski, Ritva (author)
- Uppsala universitet,Institutionen för kirurgiska vetenskaper,Anaesthesiology and Intensive Care
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- Lagrange, A. (author)
- Uppsala universitet,Institutionen för kirurgiska vetenskaper,Anaesthesiology and Intensive Care
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- Nordgren, Anders (author)
- Uppsala universitet,Institutionen för kirurgiska vetenskaper,Anaesthesiology and Intensive Care
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- Basu, Samar (author)
- Uppsala universitet,Institutionen för folkhälso- och vårdvetenskap,Clinical Nutrition
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(creator_code:org_t)
- 2003
- 2003
- English.
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In: Critical Care Medicine. - 0090-3493 .- 1530-0293. ; 31:6, s. 1780-1785
- Related links:
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http://www.ncbi.nlm....
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- OBJECTIVE: To evaluate the early myocardial biochemical inflammatory response with the microdialysis technique during porcine endotoxemia and to simultaneously monitor systemic hemodynamics. DESIGN: Prospective, randomized, placebo-controlled trial with parallel groups. SETTING: Animal research laboratory at the University Hospital of Uppsala, Sweden. SUBJECTS: Thirteen piglets aged 12-14 wks receiving general anesthesia. INTERVENTIONS: After thoracotomy and the insertion of microdialysis probes in standardized locations in the left ventricle of the heart and in the quadriceps muscle, seven pigs received a continuous infusion of endotoxin, initiating a severe endotoxemic shock. Six pigs received saline instead of endotoxin. MEASUREMENTS AND MAIN RESULTS: Endotoxemia caused a rapid and pronounced elevation of a metabolite obtained from prostaglandin degradation, 15-keto-dihydro-PGF(2alpha), in myocardial microdialysate fluid being specific of cyclooxygenase (COX)-mediated inflammation (p <.001 vs. saline-infused controls). Simultaneously, we observed a decrease in left ventricular stroke work index in the endotoxemic pigs (p <.01 vs. saline-infused controls). Endotoxemia did not alter 15-keto-dihydro-PGF(2alpha) levels in quadriceps muscle. Endotoxemia caused increases in taurine, hypoxanthine, and magnesium in myocardial microdialysate (p <.05 vs. saline-infused controls), whereas the contents of pyruvate, lactate, inosine, adenosine, and calcium were not significantly changed. CONCLUSION: Endotoxemia induced a myocardial COX-mediated inflammation without signs of ischemia. In parallel, a depletion of myocardial energy substrates and a deterioration in myocardial performance were seen.
Keyword
- Analysis of Variance
- Animals
- Dinoprost/*analogs & derivatives/metabolism
- Endotoxemia/immunology
- Female
- Hemodynamic Processes/*immunology
- Inflammation/*metabolism/microbiology
- Male
- Microdialysis
- Myocardium/immunology/*metabolism
- Prostaglandin-Endoperoxide Synthase/immunology/*metabolism
- Random Allocation
- Research Support; Non-U.S. Gov't
- Shock; Septic/*immunology
- Swine
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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