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- Andersson, MargarethaUppsala universitet,Institutionen för ytbioteknik med Centrum för ytbioteknik,Ytbioteknik,Institutionen för fysikalisk och analytisk kemi (author)
Characterization of Surface-Modified Nanoparticles for in Vivo Biointeraction. A Sedimentation Field Flow Fractionation Study
- Article/chapterEnglish2005
Publisher, publication year, extent ...
- 2005
- printrdacarrier
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- LIBRIS-ID:oai:DiVA.org:uu-74523
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-74523URI
Supplementary language notes
- Language:English
- Summary in:English
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- Sedimentation field flow fractionation (SdFFF) is an emerging high-performance analytical tool for separation and determination of size and adsorption characteristics of colloidal particles. This study demonstrates how SdFFF can be used to characterize nanoparticles prepared for in vivo applications including (1) the quantification of polymer uptake on nanoparticles where surface coverage is crucial and (2) the coupling of cell adhesive peptides containing the Arg-Gly-Asp motif (RGD). Quantitative information about polymer adhesion in order to prepare a bioinert surface and an accurate determination of ligand uptake are both of obvious importance for the understanding of, for example, relations between the number of attached molecules for biointeraction and an observed therapeutic effect. In addition, the present work highlights the necessity to perform careful characterization of commercially available particulate starting materials, in terms of size and polydispersity, prior to biological experimentation.
Subject headings and genre
- NATURVETENSKAP Kemi Fysikalisk kemi hsv//swe
- NATURAL SCIENCES Chemical Sciences Physical Chemistry hsv//eng
- FFF
- nanoparticle
- surface
- modification
- RGD
- collodial
- particles
- analysis
- quantification
- polymer
- uptake
- surface
- ligand
- biotechnology
- Physical chemistry
- Fysikalisk kemi
Added entries (persons, corporate bodies, meetings, titles ...)
- Fromell, KarinUppsala universitet,Institutionen för ytbioteknik med Centrum för ytbioteknik,Ytbioteknik,Institutionen för fysikalisk och analytisk kemi(Swepub:uu)kahul103 (author)
- Gullberg, ElisabetUppsala universitet,Institutionen för farmaci,Ytbioteknik (author)
- Artursson, PerUppsala universitet,Institutionen för farmaci,Ytbioteknik(Swepub:uu)perartur (author)
- Caldwell, KarinUppsala universitet,Institutionen för ytbioteknik med Centrum för ytbioteknik,Ytbioteknik,Institutionen för fysikalisk och analytisk kemi(Swepub:uu)karicald (author)
- Uppsala universitetInstitutionen för ytbioteknik med Centrum för ytbioteknik (creator_code:org_t)
Related titles
- In:Analytical Chemistry77, s. 5488-5493
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