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Efficient enrichmen...
Efficient enrichment of intact phosphorylated proteins by modified immobilized metal-affinity chromatography
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- Dubrovska, Anna (author)
- Uppsala universitet,Ludwiginstitutet för cancerforskning
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- Souchelnytskyi, Serhiy (author)
- Karolinska Institutet,Uppsala universitet,Ludwiginstitutet för cancerforskning
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(creator_code:org_t)
- Wiley, 2005
- 2005
- English.
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In: Proteomics. - : Wiley. - 1615-9853 .- 1615-9861. ; 5:18, s. 4678-4683
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https://urn.kb.se/re...
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Abstract
Subject headings
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- Phosphoproteome studies are hampered by the lack of methods which allow a comprehensive and fast analysis of intact phosphoproteins. Here we describe an immobilized metal-affinity chromatography (IMAC)-based technique for the enrichment of phosphorylated proteins, which allows recovery of up to 90% of phosphoproteins. This technique is compatible with 2-DE and can be applied to cultured cells and tissues.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- MEDICINE
- MEDICIN
- Cell and molecular biology
- Cell- och molekylärbiologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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