A clinical single-pass perfusion investigation of the dynamic in vivo secretory response to a dietary meal in human proximal small intestine

Purpose To investigate the gastrointestinal secretory and enzymatic responses to a liquid meal during in vivo perfusion of the proximal human jejunum. Methods Human intestinal fluid was collected from the proximal jejunum by single-pass in vivo perfusion (Loc-I-Gut). The fluid was quantitatively collected at 10-min intervals during 90 min while perfusing a nutritional drink at 2 mL/min. Quantification of lipids in the fluid leaving the segment was performed by using novel chromatographic methods. Results The overall bile acid concentration varied between 0.5 and 8.6 mM with a peak level 40 min after the start of the liquid meal perfusion. The total concentration of phospholipids was between 0.1 and 3.9 mM and there was a rapid degradation of phosphatidylcholine to lysophosphatidylcholine. The tri-, di-, monoglycerides and free fatty acid levels increased sharply in the beginning and reached steady-state levels between 7 and 9.5 mM. Conclusions There is a rapid secretion of bile in response to food. Most of the dietary lipids are found in the form of their degradation products in vivo in human jejunum. This novel in vivo characterization, based on direct and high-recovery sampling of intestinal fluids, forms a basis for further development of improved in vitro drug dissolution test media.

MEDICIN OCH HÄLSOVETENSKAP Medicinska och farmaceutiska grundvetenskaper Farmaceutiska vetenskaper hsv//swe
MEDICAL AND HEALTH SCIENCES Basic Medicine Pharmaceutical Sciences hsv//eng
Adult
Algorithms
Area Under Curve
Bile Acids and Salts/metabolism
Body Fluids/metabolism
Chromatography; High Pressure Liquid
Eating/*physiology
Enzyme Inhibitors/pharmacology
Female
Humans
Intestine; Small/physiology/*secretion
Light
Lipase/antagonists & inhibitors
Lipid Metabolism/physiology
Male
Online Systems
Perfusion
Phospholipids/metabolism
Scattering; Radiation
PHARMACY
FARMACI

Nilsson, Ralf G (author)
Hansson, Göran I (author)
Löfgren, Lars J (author)
Libäck, Fredrik (author)
Knutson, LarsUppsala universitet,Institutionen för kirurgiska vetenskaper,Loc-I-Gut-gruppen(Swepub:uu)laknu255 (author)
Abrahamsson, Bertil (author)
Lennernäs, HansUppsala universitet,Institutionen för farmaci(Swepub:uu)hanslenn (author)
Uppsala universitetInstitutionen för farmaci (creator_code:org_t)

In:Pharmaceutical research: Springer Science and Business Media LLC23:4, s. 742-7510724-87411573-904X

By the author/editor: Persson, Eva M; Nilsson, Ralf G; Hansson, Göran I; Löfgren, Lars J; Libäck, Fredrik; Knutson, Lars; show more...; Abrahamsson, Ber ...; Lennernäs, Hans; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Basic Medicine; and Pharmaceutical S ...

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