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Strong binding of bioactive BMP-2 to nanocrystalline diamond by physisorption
- Steinmüller-Nethl, Doris (author)
- Kloss, Frank (author)
- Najam-Ul-Haq, Muhammed (author)
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- Rainer, Matthias (author)
- Linsmeier, Christian (author)
- Köhler, Gottfried (author)
- Fehrer, Christine (author)
- Lepperdinger, Günter (author)
- Liu, Xianjie (author)
- Memmel, Norbert (author)
- Bertel, Erminald (author)
- Huck, Christian W. (author)
- Gassner, Robert (author)
- Bonn, Günther (author)
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- (creator_code:org_t)
- Elsevier BV, 2006
- 2006
- English.
- In: Biomaterials. - : Elsevier BV. - 0142-9612. ; 27, s. 4547-4556
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Nano-crystalline diamond (NCD)-coated surfaces were efficiently functionalized with bone morphogenetic protein-2 (BMP-2) by means of physisorption. Due to their randomly oriented texture, NCD-coated surfaces appear to bind complex molecules firmly. Applying various highly sensitive analytical methods, the interaction was found extremely stable. The strength of the experimentally measured adherence between BMP-2 and NCD was further corroborated by theoretical calculations. Oxygen treatment rendered NCD hydrophilic by the appearance of surface oxygen containing groups. This particular NCD surface exhibited even higher binding energies towards BMP-2 than the hydrophobic surface, and this surface was also favoured by cultured cells. Most importantly in this context, bound BMP-2 was found fully active. When cultured on BMP-2-treated NCD, osteosarcoma cells strongly up-regulated alkaline phosphatase, a specific marker for osteogenic differentiation. Hence, this simple method will allow generating highly versatile surfaces with complex biomimetic coatings, essentials for novel medical devices and implants as well as for innovative scaffolds in tissue engineering.
Subject headings
- NATURVETENSKAP -- Kemi -- Oorganisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Inorganic Chemistry (hsv//eng)
Keyword
- Physisorption
- BMP-2
- Bone tissue engineering
- Nanocrystalline
- Diamond
- Inorganic chemistry
- Oorganisk kemi
Publication and Content Type
- ref (subject category)
- art (subject category)
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