onr:"swepub:oai:DiVA.org:uu-89155"
Search: onr:"swepub:oai:DiVA.org:uu-89155" > Comparison of growt...
- 1 of 1
- Previous record
- Next record
- To hitlist
Comparison of growth-inhibitory agents by fluorescence imaging of human skin re-epithelialization in vitro
-
- Forsberg, Sofi (author)
- Uppsala universitet,Dermatologi och venereologi
-
- Saarialho-Kere, Ulpu (author)
- Karolinska Institutet
-
- Rollman, Ola (author)
- Uppsala universitet,Dermatologi och venereologi
- (creator_code:org_t)
- Medical Journals Sweden AB, 2006
- 2006
- English.
- In: Acta Dermato-Venereologica. - : Medical Journals Sweden AB. - 0001-5555 .- 1651-2057. ; 86:4, s. 292-299
- Journal article (peer-reviewed)
Abstract
Subject headings
Close
- Drug screening procedures should preferably utilize experimental settings mimicking the in vivo situation. The aim of this study was to evaluate a skin explant model as a tool to identify topical agents with anti-proliferative properties in human epidermis. Re-epithelialization was initiated from a skin punch biopsy explanted onto de-epidermized dermis and cultured at the air-liquid interface in the presence of the epidermal growth factor receptor kinase inhibitor PKI166, tacrolimus or established topical anti-psoriatic drugs: betamethasone, calcipotriol, dithranol and tazarotene. Neo-epidermal extension was traced by fluorescence microscopy prior to histomorphometric analysis. PKI166 at 1 mu M decreased the mean radial outgrowth rate (-19%), frequency of BrdU-positive (-37%) and laminin 5-positive (-45%) cells, indicating reduced proliferation and migration of neo-epidermal keratinocytes. However, the papillomatosis index and epithelial thickness were not significantly affected. Calcipotriol at 1 mu M had a similar effect on the outgrowth rate (-15%) and fraction of laminin 5-stained keratinocytes (-40%). Furthermore, calcipotriol significantly reduced mean neo-epidermal thickness. Equimolar concentrations of the other test compounds had no apparent effect on histology or outgrowth parameters. This study exemplifies the versatility of combined dynamic and morphological analysis and emphasizes the potential of epidermal growth factor receptor-directed inhibition in hyperproliferative disorders of the epidermis.
Keyword
- epidermal growth factor
- tyrosine kinase inhibitors
- proliferation
- epidermis
- fluorescent tracer
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
- Acta Dermato-Venereologica (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Forsberg, Sofi
- Saarialho-Kere, ...
- Rollman, Ola
- Articles in the publication
- Acta Dermato-Ven ...
- By the university
- Uppsala University
- Karolinska Institutet
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
