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  • Sundin, JohannaUppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi (author)

High yield direct 76Br-bromination of monoclonal antibodies using Cloramine-T

  • Article/chapterEnglish1999

Publisher, publication year, extent ...

  • 1999
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-89743
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-89743URI
  • https://doi.org/10.1016/S0969-8051(99)00076-1DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean +/- max error) of 77 +/- 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean +/- max error) of 75 +/- 3%, 63 +/- 4%, and 73 +/- 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.

Subject headings and genre

  • 76Br
  • Radiobromination
  • PET
  • CAT-labeling
  • Monoclonal antibodies
  • A33
  • MEDICINE
  • MEDICIN

Added entries (persons, corporate bodies, meetings, titles ...)

  • Tolmachev, Vladimir (author)
  • Koziorowski, Jacek (author)
  • Carlsson, Jörgen (author)
  • Lundqvist, Hans (author)
  • Welt, Sydney (author)
  • Larson, Steven (author)
  • Sundin, Anders (author)
  • Uppsala universitetInstitutionen för onkologi, radiologi och klinisk immunologi (creator_code:org_t)

Related titles

  • In:Nuclear Medicine and Biology26:8, s. 923-9290969-80511872-9614

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