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High yield direct 7...
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Sundin, JohannaUppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
(author)
High yield direct 76Br-bromination of monoclonal antibodies using Cloramine-T
- Article/chapterEnglish1999
Publisher, publication year, extent ...
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-89743
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-89743URI
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https://doi.org/10.1016/S0969-8051(99)00076-1DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Monoclonal antibody (MAb) A33 was labeled with the positron emitter 76Br (T(1/2) = 16.2 h). Direct labeling was done using the conventional chloramine-T method. After optimization of the labeling conditions, a maximum yield (mean +/- max error) of 77 +/- 2% was obtained at pH 6.8. In vitro binding of 76Br-A33 to SW1222 colonic cancer cells showed that the immunoreactivity was retained. Also, the MAbs 38S1 and 3S193 and the peptide hEGF were 76Br-labeled, resulting in labeling yields (mean +/- max error) of 75 +/- 3%, 63 +/- 4%, and 73 +/- 0.1%, respectively. We conclude that antibodies and peptides can be labeled conveniently with 76Br for the purpose of whole-body tumour imaging by positron emission tomography.
Subject headings and genre
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76Br
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Radiobromination
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PET
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CAT-labeling
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Monoclonal antibodies
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A33
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MEDICINE
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MEDICIN
Added entries (persons, corporate bodies, meetings, titles ...)
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Tolmachev, Vladimir
(author)
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Koziorowski, Jacek
(author)
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Carlsson, Jörgen
(author)
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Lundqvist, Hans
(author)
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Welt, Sydney
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Larson, Steven
(author)
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Sundin, Anders
(author)
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Uppsala universitetInstitutionen för onkologi, radiologi och klinisk immunologi
(creator_code:org_t)
Related titles
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In:Nuclear Medicine and Biology26:8, s. 923-9290969-80511872-9614
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