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Protein detection using proximity-dependent DNA ligation assays

Fredriksson, Simon (author)
Uppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory
Gullberg, Mats (author)
Uppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory
Jarvius, Jonas (author)
Uppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory
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Olsson, Charlotta (author)
Uppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory
Pietras, Kristian (author)
Uppsala universitet,Ludwiginstitutet för cancerforskning
Gustafsdottir, Sigrún Margrét (author)
Uppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory
Östman, Arne (author)
Uppsala universitet,Ludwiginstitutet för cancerforskning
Landegren, Ulf (author)
Uppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory
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 (creator_code:org_t)
Springer Science and Business Media LLC, 2002
2002
English.
In: Nature Biotechnology. - : Springer Science and Business Media LLC. - 1087-0156 .- 1546-1696. ; 20:5, s. 473-477
Related links:
https://urn.kb.se/re...
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https://doi.org/10.1...
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  • Journal article (peer-reviewed)
Abstract Subject headings
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  • The advent of in vitro DNA amplification has enabled rapid acquisition of genomic information. We present here an analogous technique for protein detection, in which the coordinated and proximal binding of a target protein by two DNA aptamers promotes ligation of oligonucleotides linked to each aptamer affinity probe. The ligation of two such proximity probes gives rise to an amplifiable DNA sequence that reflects the identity and amount of the target protein. This proximity ligation assay detects zeptomole (40 x 10(-21) mol) amounts of the cytokine platelet-derived growth factor (PDGF) without washes or separations, and the mechanism can be generalized to other forms of protein analysis.

Keyword

Animals
Base Sequence
Chemistry; Clinical/*methods
DNA/*metabolism
Dose-Response Relationship; Drug
Enzyme-Linked Immunosorbent Assay
Humans
Molecular Sequence Data
Oligonucleotides/*metabolism
Platelet-Derived Growth Factor/*analysis/pharmacology
Proteins/*analysis
Sensitivity and Specificity
Thrombin/pharmacology
Time Factors

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ref (subject category)
art (subject category)

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