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  • Fredriksson, SimonUppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory (author)

Protein detection using proximity-dependent DNA ligation assays

  • Article/chapterEnglish2002

Publisher, publication year, extent ...

  • Springer Science and Business Media LLC,2002
  • printrdacarrier

Numbers

  • LIBRIS-ID:oai:DiVA.org:uu-90115
  • https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-90115URI
  • https://doi.org/10.1038/nbt0502-473DOI

Supplementary language notes

  • Language:English
  • Summary in:English

Part of subdatabase

Classification

  • Subject category:ref swepub-contenttype
  • Subject category:art swepub-publicationtype

Notes

  • The advent of in vitro DNA amplification has enabled rapid acquisition of genomic information. We present here an analogous technique for protein detection, in which the coordinated and proximal binding of a target protein by two DNA aptamers promotes ligation of oligonucleotides linked to each aptamer affinity probe. The ligation of two such proximity probes gives rise to an amplifiable DNA sequence that reflects the identity and amount of the target protein. This proximity ligation assay detects zeptomole (40 x 10(-21) mol) amounts of the cytokine platelet-derived growth factor (PDGF) without washes or separations, and the mechanism can be generalized to other forms of protein analysis.

Subject headings and genre

  • Animals
  • Base Sequence
  • Chemistry; Clinical/*methods
  • DNA/*metabolism
  • Dose-Response Relationship; Drug
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Molecular Sequence Data
  • Oligonucleotides/*metabolism
  • Platelet-Derived Growth Factor/*analysis/pharmacology
  • Proteins/*analysis
  • Sensitivity and Specificity
  • Thrombin/pharmacology
  • Time Factors

Added entries (persons, corporate bodies, meetings, titles ...)

  • Gullberg, MatsUppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory (author)
  • Jarvius, JonasUppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory (author)
  • Olsson, CharlottaUppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory (author)
  • Pietras, KristianUppsala universitet,Ludwiginstitutet för cancerforskning (author)
  • Gustafsdottir, Sigrún MargrétUppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory (author)
  • Östman, ArneUppsala universitet,Ludwiginstitutet för cancerforskning (author)
  • Landegren, UlfUppsala universitet,Institutionen för genetik och patologi,Rudbeck Laboratory(Swepub:uu)ulfland (author)
  • Uppsala universitetInstitutionen för genetik och patologi (creator_code:org_t)

Related titles

  • In:Nature Biotechnology: Springer Science and Business Media LLC20:5, s. 473-4771087-01561546-1696

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