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Development of EGF-...
Development of EGF-conjugated liposomes for targeted delivery of boronated DNA-binding agents
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- Bohl Kullberg, Erika (author)
- Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap
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- Bergstrand, Nill (author)
- Uppsala universitet,Fysikalisk-kemiska institutionen
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- Carlsson, Jörgen (author)
- Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap
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- Edwards, Katarina (author)
- Uppsala universitet,Fysikalisk-kemiska institutionen
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- Johnsson, Markus (author)
- Uppsala universitet,Fysikalisk-kemiska institutionen
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- Sjöberg, Stefan (author)
- Uppsala universitet,Organisk kemi
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- Gedda, Lars (author)
- Uppsala universitet,Enheten för biomedicinsk strålningsvetenskap
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(creator_code:org_t)
- 2002-05-10
- 2002
- English.
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In: Bioconjugate chemistry. - : American Chemical Society (ACS). - 1043-1802 .- 1520-4812. ; 13:4, s. 737-743
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Liposomes are of interest as drug delivery tools for therapy of cancer and infectious diseases. We investigated conjugation of epidermal growth factor, EGF, to liposomes using the micelle-transfer method. EGF was conjugated to the distal end of PEG−DSPE lipid molecules in a micellar solution and the EGF−PEG−DSPE lipids were then transferred to preformed liposomes, either empty or containing the DNA-binding compound, water soluble acridine, WSA. We found that the optimal transfer conditions were a 1-h incubation at 60 °C. The final conjugate, 125I-EGF−liposome−WSA, contained approximately 5 mol % PEG, 10−15 EGF molecules at the liposome surface, and 104 to 105 encapsulated WSA molecules could be loaded. The conjugate was shown to have EGF-receptor-specific cellular binding in cultured human glioma cells.
Subject headings
- NATURVETENSKAP -- Kemi -- Organisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Organic Chemistry (hsv//eng)
Publication and Content Type
- ref (subject category)
- art (subject category)
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