onr:"swepub:oai:DiVA.org:uu-90900"
Search: onr:"swepub:oai:DiVA.org:uu-90900" > High-resolution qua...
- 1 of 1
- Previous record
- Next record
- To hitlist
- Castensson, AnjaUppsala universitet,Evolutionsbiologi (author)
High-resolution quantification of specific mRNA levels in human brain autopsies and biopsies
- Article/chapterEnglish2000
Publisher, publication year, extent ...
- 2000-08-01
- Cold Spring Harbor Laboratory,2000
- printrdacarrier
Numbers
- LIBRIS-ID:oai:DiVA.org:uu-90900
- https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-90900URI
- https://doi.org/10.1101/gr.10.8.1219DOI
Supplementary language notes
- Language:English
- Summary in:English
Part of subdatabase
- SwePubSwePub
Classification
Notes
- Quantification of mRNA levels in human cortical brain biopsies and autopsies was performed using a fluorogenic 5' nuclease assay. The reproducibility of the assay using replica plates was 97%-99%. Relative quantities of mRNA from 16 different genes were evaluated using a statistical approach based on ANCOVA analysis. Comparison of the relative mRNA levels between two groups of samples with different time postmortem revealed unchanged relative expression levels for most genes. Only CYP26A1 mRNA levels showed a significant decrease with prolonged time postmortem (p = 0.00004). Also, there was a general decrease in measured mRNA levels for all genes in autopsies compared to biopsies; however, on comparing mRNA levels after adjusting with reference genes, no significant differences were found between mRNA levels in autopsies and biopsies. This observation indicates that studies of postmortem material can be performed to reveal the relative in vivo mRNA levels of genes. Power calculations were done to determine the number of individuals necessary to detect differences in mRNA levels of 1.5-fold to tenfold using the strategy described here. This analysis showed that samples from at least 50 individuals per group, patients and controls, are required for high-resolution ( approximately twofold changes) differential expression screenings in the human brain. Experiments done on ten individuals per group will result in a resolution of approximately fivefold changes in expression levels. In general, the sensitivity and resolution of any differential expression study will depend on the sample size used and the between-individual variability of the genes analyzed.
Subject headings and genre
- NATURVETENSKAP Biologi Genetik hsv//swe
- NATURAL SCIENCES Biological Sciences Genetics hsv//eng
- Genetics
- Genetik
Added entries (persons, corporate bodies, meetings, titles ...)
- Emilsson, LinaUppsala universitet,Evolutionsbiologi (author)
- Preece, Paul (author)
- Jazin, ElenaUppsala universitet,Evolutionsbiologi(Swepub:uu)elenjazi (author)
- Uppsala universitetEvolutionsbiologi (creator_code:org_t)
Related titles
- In:Genome Research: Cold Spring Harbor Laboratory10:8, s. 1219-291088-90511549-5469
Internet link
Find in a library
- Genome Research (Search for host publication in LIBRIS)
To the university's database
- 1 of 1
- Previous record
- Next record
- To hitlist
Find more in SwePub
- By the author/editor
- Castensson, Anja
- Emilsson, Lina
- Preece, Paul
- Jazin, Elena
- About the subject
-
- NATURAL SCIENCES
- NATURAL SCIENCES
- and Biological Scien ...
- and Genetics
- Articles in the publication
- Genome Research
- By the university
- Uppsala University
Search outside SwePub
- Extend your search to:
- Google Book Search
- Google Scholar
Kungliga biblioteket hanterar dina personuppgifter i enlighet med EU:s dataskyddsförordning (2018), GDPR. Läs mer om hur det funkar här.
Så här hanterar KB dina uppgifter vid användning av denna tjänst.
- Copyright © LIBRIS - National Library Systems
- LIBRIS.kb.se
