Search: onr:"swepub:oai:DiVA.org:uu-92063" >
Bacteria with incre...
-
Gustafsson, IngegerdUppsala universitet,Klinisk bakteriologi
(author)
Bacteria with increased mutation frequency and antibiotic resistance are enriched in the commensal flora of patients with high antibiotic usage
- Article/chapterEnglish2003
Publisher, publication year, extent ...
-
2003-09-01
-
Oxford University Press (OUP),2003
-
printrdacarrier
Numbers
-
LIBRIS-ID:oai:DiVA.org:uu-92063
-
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-92063URI
-
https://doi.org/10.1093/jac/dkg427DOI
-
http://kipublications.ki.se/Default.aspx?queryparsed=id:19603667URI
Supplementary language notes
-
Language:English
-
Summary in:English
Part of subdatabase
Classification
-
Subject category:ref swepub-contenttype
-
Subject category:art swepub-publicationtype
Notes
-
BACKGROUND: We examined how prolonged antibiotic treatment affected the resistance and mutation frequency of human microflora isolated from intestine (Escherichia coli, enterococci spp.), pharynx (alpha-streptococci) and nostril (coagulase-negative staphylococci, CoNS).METHODS: Samples were collected from patients at the Center of Cystic Fibrosis (n=18) and the haematology ward (n=18) of the University Hospital, Uppsala, Sweden. The individually used amount of antibiotics for 1 year was recorded as the defined daily dose (DDD). Primary health care patients (n=30), with no antibiotic treatment for 1 year before sampling, were used as controls. Three isolates of each bacterium from each patient were examined. Antibiotic susceptibilities were determined by disc diffusion. Mutation frequencies to rifampicin resistance were measured on 30 independent cultures of each bacterial species from each individual by plating on rifampicin agar plates. For alpha-streptococci the mutation frequency to streptomycin resistance was also determined.RESULTS: Isolates from patients with high antibiotic use showed a pronounced shift towards increased resistance and a small but significant increase in the mutation frequency compared with isolates from the controls. For E. coli, enterococci and CoNS the increase in geometric mean mutation frequency in the patient group was 3-, 1.8- and 1.5-fold, respectively (P values 0.0001, 0.016 and 0.012). For alpha-streptococci there was a significant difference in geometric mean mutation frequency between patient and control groups for streptomycin resistance (P=0.024) but not for rifampicin resistance (P=0.74).CONCLUSIONS: High antibiotic use selected for commensals with highly increased resistance and a slight increase in mutation frequency.
Added entries (persons, corporate bodies, meetings, titles ...)
-
Sjölund, MariaUppsala universitet,Klinisk bakteriologi
(author)
-
Torell, ErikUppsala universitet,Infektionssjukdomar(Swepub:uu)eriktore
(author)
-
Johannesson, MarieUppsala universitet,Institutionen för kvinnors och barns hälsa(Swepub:uu)marjohan
(author)
-
Engstrand, LarsKarolinska Institutet,Uppsala universitet,Klinisk bakteriologi,Antibiotic Research Unit(Swepub:uu)larseng
(author)
-
Cars, OttoUppsala universitet,Klinisk bakteriologi,Antibiotic Research Unit(Swepub:uu)ottocars
(author)
-
Andersson, Dan I.
(author)
-
Uppsala universitetKlinisk bakteriologi
(creator_code:org_t)
Related titles
-
In:Journal of Antimicrobial Chemotherapy: Oxford University Press (OUP)52:4, s. 645-6500305-74531460-2091
Internet link
Find in a library
To the university's database