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cDNA cloning and ex...
cDNA cloning and expression of UDP-glucose dehydrogenase from bovine kidney
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- Lind, Thomas (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Falk, Elisabet (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Hjertson, Eva (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Kusche-Gullberg, Marion (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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- Lidholt, Kerstin (author)
- Uppsala universitet,Institutionen för medicinsk biokemi och mikrobiologi
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(creator_code:org_t)
- 1999
- 1999
- English.
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In: Glycobiology. - 0959-6658 .- 1460-2423. ; 9:6, s. 595-600
- Related links:
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https://urn.kb.se/re...
Abstract
Subject headings
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- We have isolated a cDNA encoding UDP-glucose dehydrogenase from a bovine kidney cDNA-library, the first mammalian cDNA clone published. [After submission of the manuscript, a study appeared describing the molecular cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes(Spicer et al., 1998).] The enzyme catalyzes the conversion of UDP-glucose to UDP-glucuronicacid, an essential precursor in glycosaminoglycan biosynthesis. The cDNA has an open reading frame of 1482 nucleotides coding for a 55 kDa protein. Expression of the enzyme in COS-7 cells showed a 3-fold increase inUDP-glucose dehydrogenase activity; also, the C-terminal 23 amino acidswas shown not to be necessary for enzyme activity. Northernblots from human and mouse tissues reveal high expression in liver and low in skeletal muscle. Human tissues have a majortranscript size of 3.2 kilobases and a minor of 2.6 whereas mousetissues have a single 2.6 kilobase transcript. We have also developed a sensitive and direct assay using UDP-[14C]Glc as a substrate for detection of small amounts of UDPGDH activity.
Keyword
- UDP-Glc dehydrogenase
- UDP-GlcA
- glycosaminoglycan
- proteoglycan
- biosynthesis
Publication and Content Type
- ref (subject category)
- art (subject category)
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