Capillary electrophoresis coupled to mass spectrometry from a polymer modified poly(dimethylsiloxane) microchip with an integrated graphite electrospray tip

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Pettersson Dahlin, AndreasUppsala universitet,Avdelningen för analytisk kemi (author)

Capillary electrophoresis coupled to mass spectrometry from a polymer modified poly(dimethylsiloxane) microchip with an integrated graphite electrospray tip

Article/chapterEnglish2005

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2005
Royal Society of Chemistry (RSC),2005
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LIBRIS-ID:oai:DiVA.org:uu-93084
https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-93084URI
https://doi.org/10.1039/b414592eDOI

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Language:English
Summary in:English

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Subject category:ref swepub-contenttype
Subject category:art swepub-publicationtype

Notes

Hybrid capillary-poly(dimethysiloxane) (PDMS) microchips with integrated electrospray ionization (ESI) tips were directly fabricated by casting PDMS in a mould. The shapes of the emitter tips were drilled into the mould, which produced highly reproducible three-dimensional tips. Due to the fabrication method of the microfluidic devices, no sealing was necessary and it was possible to produce a perfect channel modified by PolyE-323, an aliphatic polyamine coating agent. A variety of different coating procedures were also evaluated for the outside of the emitter tip. Dusting graphite on a thin unpolymerised PDMS layer followed by polymerisation was proven to be the most suitable procedure. The emitter tips showed excellent electrochemical properties and durabilities. The coating of the emitter was eventually passivated, but not lost, and could be regenerated by electrochemical means. The excellent electrochemical stability was further confirmed in long term electrospray experiments, in which the emitter sprayed continuously for more than 180 h. The PolyE-323 was found suitable for systems that integrate rigid fused silica and soft PDMS technology, since it simply could be applied successfully to both materials. The spray stability was confirmed from the recording of a total ion chromatogram in which the electrospray current exhibited a relative standard deviation of 3.9% for a 30 min run. CE-ESI-MS separations of peptides were carried out within 2 min using the hybrid PDMS chip resulting in similar efficiencies as for fused silica capillaries of the same length and thus with no measurable band broadening effects, originating from the PDMS emitter.

Subject headings and genre

NATURVETENSKAP Kemi Analytisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Analytical Chemistry hsv//eng
NATURVETENSKAP Kemi Oorganisk kemi hsv//swe
NATURAL SCIENCES Chemical Sciences Inorganic Chemistry hsv//eng

Added entries (persons, corporate bodies, meetings, titles ...)

Wetterhall, MagnusUppsala universitet,Avdelningen för analytisk kemi(Swepub:uu)mwe08517 (author)
Liljegren, GustavUppsala universitet,Avdelningen för analytisk kemi (author)
Bergström, Sara K.Uppsala universitet,Avdelningen för analytisk kemi(Swepub:uu)saber676 (author)
Andrén, PerUppsala universitet,Institutionen för farmaceutisk biovetenskap(Swepub:uu)perandre (author)
Nyholm, LeifUppsala universitet,Materialkemi GU(Swepub:uu)lny18455 (author)
Markides, Karin EUppsala universitet,Avdelningen för analytisk kemi (author)
Bergquist, JonasUppsala universitet,Avdelningen för analytisk kemi(Swepub:uu)jbe25356 (author)
Uppsala universitetAvdelningen för analytisk kemi (creator_code:org_t)

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In:The Analyst: Royal Society of Chemistry (RSC)130:2, s. 193-1990003-26541364-5528

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NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Analytical Chemi ...

NATURAL SCIENCES: NATURAL SCIENCES; and Chemical Science ...; and Inorganic Chemis ...

Articles in the publication: The Analyst

By the university: Uppsala University

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