Search: onr:"swepub:oai:DiVA.org:uu-9369" >
Microencapsulation ...
Microencapsulation of Pancreatic Islets : A Non-Vascularised Transplantation Model
-
- Bohman, Sara, 1981- (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
- Andersson, Arne, Professor (thesis advisor)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
- King, Aileen, Dr (thesis advisor)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
show more...
-
- Sandler, Stellan, Professor (thesis advisor)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
-
- Montanya, Eduard, Dr (opponent)
- Hospital Universitari Bellvitge, Universitat de Barcelona
-
show less...
-
(creator_code:org_t)
- ISBN 9789155473389
- Uppsala : Acta Universitatis Upsaliensis, 2008
- English 39 s.
-
Series: Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Medicine, 1651-6206 ; 396
- Related links:
-
https://uu.diva-port... (primary) (Raw object)
-
show more...
-
https://uu.diva-port... (primary) (Raw object)
-
https://uu.diva-port...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Transplantation of pancreatic islets is a potential treatment of type 1 diabetes that aims to restore normal blood glucose control. By encapsulating the islets in alginate, they can be protected from rejection. The aim of this thesis was to study the biology of encapsulated islets and to use the technique of microencapsulation to study the effect of transplantation in a system that is separated from direct contact with the vascular system and the host tissue at the transplantation site.Encapsulated islets can effectively reverse hyperglycaemia after transplantation into the peritoneal cavity of diabetic mice. A period of culture before encapsulation and transplantation did not affect their insulin release or curative capability. Pre-culture with exendin-4 improved insulin secretion, but not to the extent that the long term outcome in our transplantation model was improved. Despite being able to reach and retain normoglycaemia, microencapsulated islets transplanted intraperitoneally decreased in size. More specifically the number of beta cells in each individual islet was decreased. However, in contrast to previous studies using non-encapsulated islets, the alpha cell number was maintained, and thus the capsule seems to protect these peripherally located and otherwise exposed cells. As the capsule also prevents revascularisation of the islets, the model was used to study the importance of vascular supply for islet amyloid formation. Islet amyloid is a possible reason for the long-term failure of transplanted islets. It is likely that their low vascular density causes a disturbed local clearance of IAPP and insulin that starts the aggregation of IAPP. Indeed, encapsulated islets had an accelerated amyloid formation compared to normal islets, and might serve as a model for further studies of this process.In conclusion, although revascularisation is not a prerequisite for islet graft function, it plays an important role for islet transplantation outcome.
Subject headings
- NATURVETENSKAP -- Biologi -- Cellbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Cell Biology (hsv//eng)
Keyword
- Microencapsulation
- Islet transplantation
- revascularisation
- alpha-cell
- exendin-4
- islet amyloid
- Cell biology
- Cellbiologi
Publication and Content Type
- vet (subject category)
- dok (subject category)
Find in a library
To the university's database