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Regulation of insul...
Regulation of insulin and glucagon secretion from rat pancreatic islets in vitro by somatostatin analogues
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- Ludvigsen, Eva (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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- Stridsberg, Mats (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Klinisk kemi
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- Taylor, John (author)
- Biomeasure, Inc./Beaufour-IPSEN Group, Milford, PA, USA
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- Culler, Michael (author)
- Biomeasure, Inc./Beaufour-IPSEN Group, Milford, PA, USA
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- Öberg, Kjell (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Onkologisk endokrinologi
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- Janson, Eva Tiensuu (author)
- Uppsala universitet,Institutionen för medicinska vetenskaper,Onkologisk endokrinologi
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- Sandler, Stellan (author)
- Uppsala universitet,Institutionen för medicinsk cellbiologi
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(creator_code:org_t)
- Elsevier BV, 2007
- 2007
- English.
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In: Regulatory Peptides. - : Elsevier BV. - 0167-0115 .- 1873-1686. ; 138:1, s. 1-9
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- Somatostatin is an inhibitor of hormone secretion through specific receptors (sst1-5). The aim of this study was to investigate the putative regulatory role of somatostatin analogues on the secretion of insulin and glucagon by rat pancreatic islets. After 48 h exposure only the non-selective agonists (somatostatin, octreotide and SOM-230) inhibited insulin accumulation. The inhibition of insulin secretion was accompanied by increased islet insulin contents. None of the analogues showed a consistent effect on the glucagon accumulation in the medium after 48 h. Since we observed a difference in the regulatory effect between the non-selective and selective analogues, combinations of selective analogues were studied. Combination of sst2 + sst5 agonists inhibited the medium insulin accumulation, while combination of sst1 + sst2 analogues caused a decrease in glucagon accumulation. After removal of somatostatin a rebound effect with increased insulin secretion were observed. This effect was reversed after 6 h. For SOM-230 insulin secretion continued to be suppressed even after the analogue was removed and returned to control values after 3 h. As for glucagon secretion there was an initial decline after culture with octreotide, while the other substances failed to induce any changes. In summary, non-selective somatostatin analogues or combinations of receptor selective analogues may cause inhibition of hormone secretion from rat pancreatic islets. For insulin and glucagon, combinations of sst2 + sst5 and sst1 + sst2, respectively may exert this effects. Thus, our data suggest that more than one sst must be involved to down-regulate islet glucagon and insulin secretion.
Keyword
- Glucagon secretion
- Insulin secretion
- Pancreatic islets
- Somatostatin analogues
- Somatostatin receptors
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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