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Cartridge-based hig...
Cartridge-based high-throughput purification of oligonucleotides for reliable oligonucleotide arrays
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- Semenyuk, Andrey (author)
- Uppsala universitet,Institutionen för genetik och patologi
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Ahnfelt, Matilda (author)
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Estmer Nilsson, Camilla (author)
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Hao, Xiao Yong (author)
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Földesi, Andras (author)
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Kao, Yu-Shu (author)
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Chen, Hong-Huei (author)
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Kao, Wei-Chen (author)
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Peck, Konan (author)
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- Kwiatkowski, Marek (author)
- Uppsala universitet,Institutionen för genetik och patologi
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(creator_code:org_t)
- Elsevier BV, 2006
- 2006
- English.
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In: Analytical Biochemistry. - : Elsevier BV. - 0003-2697 .- 1096-0309. ; 356:1, s. 132-141
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- A novel, cartridge-based procedure for the efficient and irreversible detritylation of oligonucleotides is reported. This method, combined with a process for the elimination of depurinated fragments produces, in a highly parallel fashion, oligonucleotides with better purity than those traditionally obtained using reversed-phase high-performance liquid chromotography purification. Our combined detritylation and purification methodology compares favorably with commercial cartridge-based purification systems. The benefits of working with pure oligonucleotides, with regard to higher signal and better signal linearity, are shown in array-based hybridization experiments.
Keyword
- oligonucleotide
- synthesis
- purification
- cartridge
- microarray
- MEDICINE
- MEDICIN
Publication and Content Type
- ref (subject category)
- art (subject category)
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To the university's database
- By the author/editor
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Semenyuk, Andrey
-
Ahnfelt, Matilda
-
Estmer Nilsson, ...
-
Hao, Xiao Yong
-
Földesi, Andras
-
Kao, Yu-Shu
-
show more...
-
Chen, Hong-Huei
-
Kao, Wei-Chen
-
Peck, Konan
-
Kwiatkowski, Mar ...
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show less...
- Articles in the publication
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Analytical Bioch ...
- By the university
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Uppsala University