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Digital Quantification using Amplified Single-Molecule Detection
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- Jarvius, Jonas (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- Melin, Jonas (author)
- Uppsala universitet,Fasta tillståndets fysik
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- Göransson, Jenny (author)
- Uppsala universitet,Institutionen för genetik och patologi
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- 2006
- 2006
- English.
- In: Nature Methods. - 1548-7091 .- 1548-7105. ; 3:9, s. 725-727
- Journal article (peer-reviewed)
Abstract
Subject headings
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- We describe a scheme for biomolecule enumeration by converting nanometer-scale specific molecular recognition events mediated by rolling-circle amplification to fluorescent micrometer-sized DNA molecules amenable to discrete optical detection. Our amplified single-molecule detection (SMD) approach preserves the discrete nature of the molecular population, allowing multiplex detection and highly precise quantification of molecules over a dynamic range of seven orders of magnitude. We apply the method for sensitive detection and quantification of the bacterial pathogen Vibrio cholerae.
Subject headings
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Biology
- Biologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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