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Species differences...
Species differences in 3-methylsulphonyl-DDE bioactivation by adrenocortical tissue
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- Lindström, Veronica (author)
- Uppsala universitet,Ekotoxikologi
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- Brandt, Ingvar (author)
- Uppsala universitet,Ekotoxikologi
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- Lindhe, Örjan (author)
- Uppsala universitet,Ekotoxikologi
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(creator_code:org_t)
- 2007-11-23
- 2008
- English.
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In: Archives of Toxicology. - : Springer Science and Business Media LLC. - 0340-5761 .- 1432-0738. ; 82:3, s. 159-163
- Related links:
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https://urn.kb.se/re...
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https://doi.org/10.1...
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Abstract
Subject headings
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- The CYP11B1-activated adrenocortical toxicant 3-methylsulphonyl-DDE (3-MeSO2-DDE) is proposed as a lead compound for an improved chemotherapy for adrenocortical carcinoma. We compared the binding of 3-MeSO2-[C-14]DDE in the adrenal cortex of four rodent species; hamster, guinea pig, mouse and rat, using a precision-cut adrenal slice culture system ex vivo. Localization and quantification of the bound radioactivity were carried out using light microscopy autoradiography and radioluminography. The results revealed major species differences since 3-MeSO2-[C-14]DDE was extensively bound to the hamster adrenal tissue while the guinea pig adrenals were devoid of binding. A high binding in mouse adrenal cortex was confirmed while binding in rat adrenal cortex was very weak. The results support previous observations that metabolic activation of 3-MeSO2-DDE is highly species dependent. Since CYP11B1 could be expressed in tissues other than the adrenal cortex, final toxicological characterization should be carried out in a species that can bioactivate this compound.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Keyword
- 3-MeSO2-DDE
- hamster
- guinea pig
- CYP450
- irreversible binding
- adrenal
- tissue-slice
- Toxicology
- Toxikologi
Publication and Content Type
- ref (subject category)
- art (subject category)
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