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A new method for in...
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Cabric, SanjaUppsala universitet,Enheten för klinisk immunologi
(author)
A new method for incorporating functional heparin onto the surface of islets of Langerhans
- Article/chapterEnglish2008
Publisher, publication year, extent ...
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Mary Ann Liebert Inc,2008
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Numbers
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LIBRIS-ID:oai:DiVA.org:uu-96504
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-96504URI
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https://doi.org/10.1089/ten.tec.2007.0312DOI
Supplementary language notes
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Language:English
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Summary in:English
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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A novel technique is described to conjugate macromolecular heparin complexes to cell surfaces. The method is based on the dual properties of avidin-expressing binding sites for both biotin and a macromolecular complex of heparin. A quartz crystal microbalance with dissipation monitoring (QCM-D) revealed sequential binding of biotin, avidin, and heparin complexes. Large particle flow cytometry confirmed functional integrity. Confocal microscopy of the heparinized islets showed evenly distributed fluorescence. An in vitro Chandler loop model demonstrated that the biocompatibility of the new method is comparable to the previous method used on artificial materials with regard to coagulation and antithrombin uptake. The technique presented allows human islets of Langerhans to successfully be covered with functional heparin as a means to reduce instant blood-mediated inflammatory reactions induced by the innate immune system.
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Eich, TorstenUppsala universitet,Enheten för klinisk immunologi
(author)
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Sanchez, JavierUppsala universitet,Enheten för klinisk immunologi(Swepub:uu)jasan991
(author)
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Nilsson, BoUppsala universitet,Enheten för klinisk immunologi(Swepub:uu)bonils
(author)
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Korsgren, OlleUppsala universitet,Enheten för klinisk immunologi(Swepub:uu)ollekors
(author)
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Larsson, RolfUppsala universitet,Enheten för klinisk immunologi(Swepub:uu)rolflars
(author)
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Uppsala universitetEnheten för klinisk immunologi
(creator_code:org_t)
Related titles
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In:Tissue Engineering. Part C, Methods: Mary Ann Liebert Inc14:2, s. 141-1471937-33841937-3392
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