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Islet Surface Hepar...
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Cabric, SanjaUppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,Ö-cellslab
(author)
Islet Surface Heparinization Prevents the Instant-Blood Mediated Inflammatory Reaction in Islet Transplantation
- Article/chapterEnglish2007
Publisher, publication year, extent ...
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American Diabetes Association,2007
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-96505
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-96505URI
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https://doi.org/10.2337/db07-0358DOI
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http://kipublications.ki.se/Default.aspx?queryparsed=id:115872795URI
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https://lup.lub.lu.se/record/686702URI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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OBJECTIVE—In clinical islet transplantation, the instant blood-mediated inflammatory reaction (IBMIR) is a major factor contributing to the poor initial engraftment of the islets. This reaction is triggered by tissue factor and monocyte chemoattractant protein (MCP)-1, expressed by the transplanted pancreatic islets when the islets come in contact with blood in the portal vein. All currently identified systemic inhibitors of the IBMIR are associated with a significantly increased risk of bleeding or other side effects. To avoid systemic treatment, the aim of the present study was to render the islet graft blood biocompatible by applying a continuous heparin coating to the islet surface.RESEARCH DESIGN AND METHODS—A biotin/avidin technique was used to conjugate preformed heparin complexes to the surface of pancreatic islets. This endothelial-like coating was achieved by conjugating barely 40 IU heparin per full-size clinical islet transplant.RESULTS—Both in an in vitro loop model and in an allogeneic porcine model of clinical islet transplantation, this heparin coating provided protection against the IBMIR. Culturing heparinized islets for 24 h did not affect insulin release after glucose challenge, and heparin-coated islets cured diabetic mice in a manner similar to untreated islets.CONCLUSIONS—This novel pretreatment procedure prevents intraportal thrombosis and efficiently inhibits the IBMIR without increasing the bleeding risk and, unlike other pretreatment procedures (e.g., gene therapy), without inducing acute or chronic toxicity in the islets.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Sanchez, JavierUppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi,Komplement och Biomaterial
(author)
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Lundgren, TorbjörnKarolinska Institutet
(author)
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Foss, Aksel
(author)
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Felldin, Marie
(author)
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Källén, RagnarLund University,Lunds universitet,Kirurgi,Forskargrupper vid Lunds universitet,Surgery,Lund University Research Groups(Swepub:lu)med-rgk
(author)
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Salmela, Kaija
(author)
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Tibell, AnnikaKarolinska Institutet
(author)
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Tufveson, GunnarUppsala universitet,Transplantationskirurgi(Swepub:uu)gutuf839
(author)
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Larsson, RolfUppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi
(author)
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Korsgren, OlleUppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi(Swepub:uu)ollekors
(author)
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Nilsson, BoUppsala universitet,Institutionen för onkologi, radiologi och klinisk immunologi(Swepub:uu)bonils
(author)
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Uppsala universitetInstitutionen för onkologi, radiologi och klinisk immunologi
(creator_code:org_t)
Related titles
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In:Diabetes: American Diabetes Association56:8, s. 2008-20150012-17971939-327X
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Cabric, Sanja
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Sanchez, Javier
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Lundgren, Torbjö ...
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Foss, Aksel
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Felldin, Marie
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Källén, Ragnar
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Salmela, Kaija
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Tibell, Annika
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Tufveson, Gunnar
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Larsson, Rolf
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Korsgren, Olle
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Nilsson, Bo
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- MEDICAL AND HEALTH SCIENCES
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Diabetes
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Uppsala University
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