Search: onr:"swepub:oai:DiVA.org:uu-96684" >
Identification and ...
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Essand, MagnusUppsala universitet,Enheten för klinisk immunologi
(author)
Identification and characterization of a novel splicing variant of vesicular monoamine transporter 1
- Article/chapterEnglish2005
Publisher, publication year, extent ...
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Bioscientifica,2005
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printrdacarrier
Numbers
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LIBRIS-ID:oai:DiVA.org:uu-96684
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https://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-96684URI
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https://doi.org/10.1677/jme.1.01875DOI
Supplementary language notes
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Language:English
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Summary in:English
Part of subdatabase
Classification
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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Vesicular monoamine transporter 1 (VMAT1) is an integral protein in the membrane of secretory vesicles of neuroendocrine and endocrine cells that allows the transport of biogenic monoamines, such as serotonin, from the cytoplasm into the secretory vesicles. The full-length VMAT1 transcript is produced from 16 exons. We have identified and characterized an alternatively spliced form of VMAT1 that lacks exon 15, the next to last exon of VMAT1. The new form was therefore denoted VMAT1Delta15. Exon 15 does not contain an even multiple of three nucleotides. As a consequence, there is a shift of reading frame, and exon 16 is translated in an alternative reading frame, yielding a novel protein with a shorter and unrelated C-terminus compared with the native VMAT1 protein. VMAT1 and VMAT1Delta15 mRNAs are simultaneously expressed in normal and neoplastic neuroendocrine cells of the GI tract. However, VMAT1 expression is always higher than VMAT1Delta15 expression. We prove that VMAT1Delta15 is not localized in large, dense core vesicles as the native form but in the endoplasmic reticulum. Furthermore, while VMAT1 can take up serotonin, VMAT1Delta15 cannot, indicating different functions for the two forms of VMAT1.
Subject headings and genre
Added entries (persons, corporate bodies, meetings, titles ...)
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Vikman, SofiaUppsala universitet,Enheten för klinisk immunologi
(author)
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Grawé, JanUppsala universitet,Institutionen för genetik och patologi
(author)
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Gedda, LarsUppsala universitet,Enheten för biomedicinsk strålningsvetenskap(Swepub:uu)larsgedd
(author)
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Hellberg, CarinaUppsala universitet,Ludwiginstitutet för cancerforskning
(author)
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Öberg, KjellUppsala universitet,Institutionen för medicinska vetenskaper,Onkologisk endokrinologi
(author)
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Tötterman, Thomas H.Uppsala universitet,Enheten för klinisk immunologi(Swepub:uu)thomtott
(author)
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Giandomenico, ValeriaUppsala universitet,Enheten för klinisk immunologi
(author)
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Uppsala universitetEnheten för klinisk immunologi
(creator_code:org_t)
Related titles
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In:Journal of Molecular Endocrinology: Bioscientifica35:3, s. 489-5010952-50411479-6813
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