Search: onr:"swepub:oai:DiVA.org:uu-97625" >
Sediment extraction...
Sediment extraction and clean-up for organic phosphorus analysis by electrospray ionization tandem mass spectrometry
-
- De Brabandere, Heidi (author)
- Uppsala universitet,Analytisk kemi
-
- Sjöberg, Per J. R. (author)
- Uppsala universitet,Analytisk kemi
-
- Danielsson, Rolf (author)
- Uppsala universitet,Analytisk kemi
-
show more...
-
- Ahlgren, Joakim (author)
- Uppsala universitet,Analytisk kemi
-
- Rydin, Emil (author)
- Uppsala universitet,Limnologi
-
- Waldebäck, Monica (author)
- Uppsala universitet,Analytisk kemi
-
show less...
-
(creator_code:org_t)
- Elsevier BV, 2008
- 2008
- English.
-
In: Talanta. - : Elsevier BV. - 0039-9140 .- 1873-3573. ; 74:5, s. 1175-1183
- Related links:
-
https://urn.kb.se/re...
-
show more...
-
https://doi.org/10.1...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- A method to prepare NaOH sediment extracts for organic P compound analysis with electrospray ionization tandem mass spectrometry (ESI-MS-MS) was developed on natural samples. Ion exchange, rotary evaporation and mass cut-off filtering proved to be suitable for sample preparation. Samples were analyzed with ESI-MS-MS, and reproducibility and repeatability of the method was calculated. In addition, 31P-nuclear magnetic resonance spectroscopy (31P NMR) was used to measure recovery of different P compound groups such as orthophosphate (Ortho-P), orthophosphate monoesters (Monoester-P), orthophosphate diesters (Diester-P) and pyrophosphates (Pyro-P). The developed sample preparation method resulted in an easy-to-spray liquid for the ESI-MS-MS instrumentation. The overall P recovery was 65% and 31P NMR showed that Diester-P, possibly in the form of DNA, was apparently lost through the filtering step most likely due to their size. Variances in the total intensities of the MS scans (relative standard deviation (R.S.D.) 35–54%) were for about 50% due to repeated MS runs. Covariances of the peaks in the MS spectra were calculated to be for about 30% due to the sample preparation procedure. Finally, with the ESI-MS-MS approach, 11 peaks in the mass spectra were found likely to represent phosphate containing compounds.
Subject headings
- NATURVETENSKAP -- Kemi -- Analytisk kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Analytical Chemistry (hsv//eng)
Keyword
- Organic phosphorus
- Sediment
- Sample preparation
- ESI-MS-MS
- 31P NMR
- Analytical chemistry
- Analytisk kemi
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
Talanta
(Search for host publication in LIBRIS)
To the university's database