Search: onr:"swepub:oai:gup.ub.gu.se/100482" >
Crystal structure o...
Crystal structure of a yeast aquaporin at 1.15 angstrom reveals a novel gating mechanism.
-
- Fischer, Gerhard, 1978 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
-
- Kosinska-Eriksson, Urszula, 1976 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
-
Aponte-Santamaría, Camilo (author)
-
show more...
-
- Palmgren, Madelene (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,University of Gothenburg
-
- Geijer, Cecilia, 1980 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,University of Gothenburg
-
- Hedfalk, Kristina, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
-
- Hohmann, Stefan, 1956 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,University of Gothenburg
-
de Groot, Bert L (author)
-
- Neutze, Richard, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för kemi,Department of Chemistry,University of Gothenburg
-
- Lindkvist-Petersson, Karin (author)
- Gothenburg University,Göteborgs universitet,Institutionen för cell- och molekylärbiologi,Department of Cell and Molecular Biology,University of Gothenburg
-
show less...
-
(creator_code:org_t)
- 2009-06-16
- 2009
- English.
-
In: PLoS biology. - : Public Library of Science (PLoS). - 1545-7885 .- 1544-9173. ; 7:6
- Related links:
-
https://doi.org/10.1...
-
show more...
-
http://dx.doi.org/10...
-
https://gup.ub.gu.se...
-
https://doi.org/10.1...
-
https://research.cha...
-
show less...
Abstract
Subject headings
Close
- Aquaporins are transmembrane proteins that facilitate the flow of water through cellular membranes. An unusual characteristic of yeast aquaporins is that they frequently contain an extended N terminus of unknown function. Here we present the X-ray structure of the yeast aquaporin Aqy1 from Pichia pastoris at 1.15 A resolution. Our crystal structure reveals that the water channel is closed by the N terminus, which arranges as a tightly wound helical bundle, with Tyr31 forming H-bond interactions to a water molecule within the pore and thereby occluding the channel entrance. Nevertheless, functional assays show that Aqy1 has appreciable water transport activity that aids survival during rapid freezing of P. pastoris. These findings establish that Aqy1 is a gated water channel. Mutational studies in combination with molecular dynamics simulations imply that gating may be regulated by a combination of phosphorylation and mechanosensitivity.
Subject headings
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
Keyword
- Aquaporins
- chemistry
- metabolism
- Biological Transport
- Computer Simulation
- Crystallography
- X-Ray
- Freezing
- Ion Channel Gating
- Microbial Viability
- Models
- Molecular
- Phosphorylation
- Pichia
- chemistry
- Protein Structure
- Secondary
- Spinacia oleracea
- chemistry
- Structural Homology
- Protein
- Tyrosine
- metabolism
- Water
- Freezing
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
To the university's database
- By the author/editor
-
Fischer, Gerhard ...
-
Kosinska-Eriksso ...
-
Aponte-Santamarí ...
-
Palmgren, Madele ...
-
Geijer, Cecilia, ...
-
Hedfalk, Kristin ...
-
show more...
-
Hohmann, Stefan, ...
-
de Groot, Bert L
-
Neutze, Richard, ...
-
Lindkvist-Peters ...
-
show less...
- About the subject
-
- NATURAL SCIENCES
-
NATURAL SCIENCES
-
and Chemical Science ...
- Articles in the publication
-
PLoS biology
- By the university
-
University of Gothenburg
-
Chalmers University of Technology