Search: onr:"swepub:oai:gup.ub.gu.se/105080" >
Comparative analysi...
Comparative analysis of human gut microbiota by barcoded pyrosequencing
-
- Andersson, A. F. (author)
- Uppsala universitet,Limnologi
-
Lindberg, M. (author)
-
Jakobsson, H. (author)
-
show more...
-
- Bäckhed, Fredrik, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för molekylär och klinisk medicin,Institute of Medicine, Department of Molecular and Clinical Medicine
-
- Nyrén, Pål (author)
- KTH,Skolan för bioteknologi (BIO)
-
- Engstrand, L. (author)
- Karolinska Institutet
-
show less...
-
(creator_code:org_t)
- Public Library of Science (PLoS), 2008
- 2008
- English.
-
In: PLoS ONE. - : Public Library of Science (PLoS). - 1932-6203. ; 3:7
- Related links:
-
https://journals.plo...
-
show more...
-
http://www.plosone.o...
-
https://gup.ub.gu.se...
-
https://urn.kb.se/re...
-
https://doi.org/10.1...
-
http://kipublication...
-
https://urn.kb.se/re...
-
show less...
Abstract
Subject headings
Close
- Humans host complex microbial communities believed to contribute to health maintenance and, when in imbalance, to the development of diseases. Determining the microbial composition in patients and healthy controls may thus provide novel therapeutic targets. For this purpose, high-throughput, cost-effective methods for microbiota characterization are needed. We have employed 454-pyrosequencing of a hyper-variable region of the 16S rRNA gene in combination with sample-specific barcode sequences which enables parallel in-depth analysis of hundreds of samples with limited sample processing. In silico modeling demonstrated that the method correctly describes microbial communities down to phylotypes below the genus level. Here we applied the technique to analyze microbial communities in throat, stomach and fecal samples. Our results demonstrate the applicability of barcoded pyrosequencing as a high-throughput method for comparative microbial ecology.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
Keyword
- Adult
- Aged
- Biopsy
- DNA Primers
- Ecology
- Endoscopy
- Genetic Techniques
- Humans
- Intestines/ microbiology
- Microbiological Techniques
- Middle Aged
- Models
- Biological
- Phylogeny
- Sequence Analysis
- DNA
- Stomach/ microbiology
- Biology
- MEDICINE
Publication and Content Type
- ref (subject category)
- art (subject category)
Find in a library
-
PLoS ONE
(Search for host publication in LIBRIS)
To the university's database