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Optimization of the...
Optimization of the detection of microbes in blood from immunocompromised patients with haematological malignancies.
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- Skovbjerg, Susann, 1973 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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- Welinder-Olsson, Christina, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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- Kondori, Nahid, 1967 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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Kjellin, Eva (author)
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- Nowrouzian, Forough, 1957 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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- Wold, Agnes E, 1955 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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- Stockelberg, Dick, 1950 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för invärtesmedicin,Institute of Medicine, Department of Internal Medicine
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Larsson, P (author)
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- Wennerås, Christine, 1963 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine
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(creator_code:org_t)
- Elsevier BV, 2009
- 2009
- English.
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In: Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases. - : Elsevier BV. - 1469-0691. ; 15:7, s. 680-3
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Abstract
Subject headings
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- The present study aimed to improve the rate of detection of blood-borne microbes by using PCRs with pan-bacterial and Candida specificity. Seventeen per cent of the blood samples (n=178) collected from 107 febrile patients with haematological malignancies were positive using standard culture (BacT/Alert system). Candida PCR was positive in 12 patients, only one of whom scored culture-positive. Bacterial PCR using fresh blood samples was often negative, but the detection rate increased when the blood was pre-incubated for 2 days. These data indicate that PCR assays might be a complement for the detection of blood-borne opportunists in immunocompromised haematology patients.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
Keyword
- Bacteremia
- epidemiology
- microbiology
- Blood
- microbiology
- Candida
- classification
- genetics
- isolation & purification
- Candida albicans
- genetics
- isolation & purification
- Candida glabrata
- genetics
- isolation & purification
- Candidiasis
- microbiology
- Culture Media
- DNA
- Bacterial
- analysis
- DNA
- Fungal
- analysis
- Fever
- Fungemia
- epidemiology
- microbiology
- Gram-Negative Bacteria
- genetics
- isolation & purification
- Gram-Positive Bacteria
- genetics
- isolation & purification
- Hematologic Neoplasms
- complications
- Humans
- Immunocompromised Host
- Neutropenia
- Opportunistic Infections
- epidemiology
- microbiology
- Polymerase Chain Reaction
- methods
Publication and Content Type
- ref (subject category)
- art (subject category)
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Skovbjerg, Susan ...
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Welinder-Olsson, ...
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Kondori, Nahid, ...
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Kjellin, Eva
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Nowrouzian, Foro ...
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Wold, Agnes E, 1 ...
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Stockelberg, Dic ...
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Larsson, P
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Wennerås, Christ ...
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- About the subject
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- MEDICAL AND HEALTH SCIENCES
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MEDICAL AND HEAL ...
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and Basic Medicine
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and Microbiology in ...
- Articles in the publication
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Clinical microbi ...
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Clinical Microbi ...
- By the university
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University of Gothenburg