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Calpain processing ...
Calpain processing of brain microtubules from the Atlantic cod, Gadus morhua.
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- Billger, Martin (author)
- Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology
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- Nilsson, E (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Karlsson, Jan-Olof, 1944 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Wallin, Margareta, 1952 (author)
- Gothenburg University,Göteborgs universitet,Zoologiska institutionen,Department of Zoology
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(creator_code:org_t)
- 1993
- 1993
- English.
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In: Molecular and cellular biochemistry. - 0300-8177. ; 121:1, s. 85-92
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Abstract
Subject headings
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- Microtubules isolated from Atlantic cod (Gadus morhua) brains retained assembly competence and ultraculture, although treatment with rabbit calpain resulted in loss of MAPs. In addition, spirals and aberrant structures formed when calpain I was activated post assembly. No such effect was seen with calpain II. Soluble fractions from cod brain were found to contain proteolytic activity that could be blocked by exogenously added calpastatin. Calpain was also isolated from cod muscle tissue with 10 times less yield, compared to rabbit lung. On the basis of Ca(2+)-requirements for activation in the mM range, electrophoretic mobility, antigenicity and hydrophobicity, we conclude that the proteolytic activity was attributable to calpain II. There was no difference in effects of rabbit and cod calpain II on cod microtubule proteins, indicating that calpain is a conserved protein. Our results suggest that calpains might be involved in the Ca(2+)-dependent irreversible regulation of cod brain microtubules.
Subject headings
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
Keyword
- Animals
- Brain Chemistry
- Calpain
- analysis
- pharmacology
- Electrophoresis
- Polyacrylamide Gel
- Fishes
- Microscopy
- Electron
- Microtubule-Associated Proteins
- metabolism
- Microtubules
- drug effects
- metabolism
- ultrastructure
- Muscles
- chemistry
- Rabbits
Publication and Content Type
- ref (subject category)
- art (subject category)
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