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Role of melanin pig...
Role of melanin pigment in expression of Vibrio cholerae virulence factors.
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- Valeru, Soni Priya (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Rompikuntal, Pramod Kumar (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Ishikawa, Takahiko (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Vaitkevicius, Karolis (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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- Sjöling, Åsa, 1968 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för mikrobiologi och immunologi,Institute of Biomedicine, Department of Microbiology and Immunology
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Dolganov, Nadia (author)
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Zhu, Jun (author)
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Schoolnik, Gary (author)
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- Wai, Sun Nyunt (author)
- Umeå universitet,Institutionen för molekylärbiologi (Medicinska fakulteten)
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(creator_code:org_t)
- American Society for Microbiology, 2009
- 2009
- English.
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In: Infection and immunity. - : American Society for Microbiology. - 1098-5522 .- 0019-9567. ; 77:3, s. 935-42
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Abstract
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- We identified the mutated gene locus in a pigment-overproducing Vibrio cholerae mutant of strain A1552. The deduced gene product is suggested to be an oxidoreductase based on partial homology to putative homogentisate 1,2-dioxygenase in Pseudomonas aeruginosa and Mesorhizobium loti, and we propose that the gene VC1345 in the V. cholerae genome be denoted hmgA in accordance with the nomenclature for other species. The hmgA::mini-Tn5 mutant showed a nonpigmented phenotype after complementation with a plasmid clone carrying the WT hmgA(+) locus. Microarray transcription analysis revealed that expression of hmgA and the neighboring genes encoding a postulated two-component sensor system was growth phase dependent. Results from quantitative reverse transcription-PCR analysis showed that hmgA operon expression was reduced in the rpoS mutant, but pigment production by the WT V. cholerae or the hmgA mutant was not detectably influenced by the stationary-phase regulator RpoS. The pigmented mutant showed increased UV resistance in comparison with the WT strain. Interestingly, the pigment-producing mutant expressed more toxin-coregulated pilus and cholera toxin than WT V. cholerae. Moreover, the hmgA mutant showed a fivefold increase in the ability to colonize the intestines of infant mice. A possible mechanism by which pigment production might cause induction of the ToxR regulon due to generation of hydrogen peroxide was supported by results from tests showing that externally supplied H(2)O(2) led to higher TcpA levels. Taken together, our findings suggest that melanin pigment formation may play a role in V. cholerae virulence factor expression.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Mikrobiologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Microbiology in the medical area (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Infektionsmedicin (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Infectious Medicine (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Immunologi inom det medicinska området (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Immunology in the medical area (hsv//eng)
Keyword
- Bacterial Proteins
- genetics
- Blotting
- Western
- DNA Transposable Elements
- Gene Expression Regulation
- Bacterial
- genetics
- Genes
- Bacterial
- genetics
- Melanins
- genetics
- metabolism
- Mutation
- Oligonucleotide Array Sequence Analysis
- Oxidoreductases
- genetics
- Reverse Transcriptase Polymerase Chain Reaction
- Sigma Factor
- genetics
- Vibrio cholerae
- genetics
- pathogenicity
- Virulence Factors
- genetics
- mannose-sensitive hemagglutinin
Publication and Content Type
- ref (subject category)
- art (subject category)
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Valeru, Soni Pri ...
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Rompikuntal, Pra ...
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Ishikawa, Takahi ...
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Vaitkevicius, Ka ...
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Sjöling, Åsa, 19 ...
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Dolganov, Nadia
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show more...
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Zhu, Jun
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Schoolnik, Gary
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Wai, Sun Nyunt
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show less...
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Infection and im ...
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University of Gothenburg
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Umeå University