Interlaboratory comparison of methodologies for the measurement of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine.

Cooke, Marcus S (author)

Barregård, Lars, 1948 (author): Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa,Institute of Medicine, School of Public Health and Community Medicine

Mistry, Vilas (author)

Potdar, Neelam (author)

Rozalski, Rafal (author)

Gackowski, Daniel (author)

Siomek, Agnieszka (author)

Foksinski, Marek (author)

Svoboda, Peter (author)

Kasai, Hiroshi (author)

Konje, Justin C (author)

Sällsten, Gerd, 1952 (author): Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för samhällsmedicin och folkhälsa,Institute of Medicine, School of Public Health and Community Medicine

Evans, Mark D (author)

Olinski, Ryszard (author)

(creator_code:org_t)

2009-03
2009
English.
In: Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals. - : Informa UK Limited. - 1366-5804. ; 14:2, s. 103-10

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Abstract Subject headings

Urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) is widely used as a marker of oxidative stress. Here we report the comparison of two, distinct chromatographic assays with an enzyme-linked immunosorbent assay (ELISA). The chromatographic assays displayed good agreement (r =:0.89, p < 0.0001), whereas there was markedly worse, albeit still significant, agreement with the ELISA (high-pressure liquid chromatography followed by gas chromatography (HPLC-GC/MS), r = 0.43; HPLC with electrochemical detection (HPLC-EC), r = 0.56; p < 0.0001). Mean values differed significantly between the chromatographic assays and the ELISA (HPLC-GC/MS 3.86, HPLC-EC 4.20, ELISA 18.70 ng mg(-1) creatinine; p < 0.0001). While it is reassuring to note good agreement between chromatographic assays, this study reveals significant short-comings in the ELISA, which brings into question its continued use in its present form.

MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Dermatologi och venereologi (hsv//swe)
MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Dermatology and Venereal Diseases (hsv//eng)

By the author/editor: Cooke, Marcus S; Barregård, Lars, ...; Mistry, Vilas; Potdar, Neelam; Rozalski, Rafal; Gackowski, Danie ...; show more...; Siomek, Agnieszk ...; Foksinski, Marek; Svoboda, Peter; Kasai, Hiroshi; Konje, Justin C; Sällsten, Gerd, ...; Evans, Mark D; Olinski, Ryszard; show less...

About the subject

MEDICAL AND HEALTH SCIENCES: MEDICAL AND HEAL ...; and Clinical Medicin ...; and Dermatology and ...

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