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Insulin-like growth...
Insulin-like growth factor-I increases astrocyte intercellular gap junctional communication and connexin43 expression in vitro.
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- Åberg, N David, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap, Sektionen för laborativ neurovetenskap,Institute of Clinical Neurosciences, Section of Experimental Neuroscience
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- Blomstrand, Fredrik, 1969 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Åberg, Maria A I, 1972 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap, Sektionen för laborativ neurovetenskap,Institute of Clinical Neurosciences, Section of Experimental Neuroscience
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- Björklund, Ulrika, 1970 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för anatomi och cellbiologi,Institute of Anatomy and Cell Biology
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- Carlsson, Björn, 1958 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för invärtesmedicin, Avdelningen för kroppssammansättning och metabolism,Institute of Internal Medicine, Dept of Body Composition and Metabolism
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- Carlsson-Skwirut, Christine (author)
- Karolinska Institutet
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- Bang, Peter (author)
- Karolinska Institutet
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- Rönnbäck, Lars, 1951 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap, Sektionen för laborativ neurovetenskap,Institute of Clinical Neurosciences, Section of Experimental Neuroscience
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- Eriksson, Peter S, 1959 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för klinisk neurovetenskap,Institute of Clinical Neurosciences
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Aberg, MAL (author)
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(creator_code:org_t)
- 2003-08-01
- 2003
- English.
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In: Journal of neuroscience research. - : Wiley. - 0360-4012. ; 74:1, s. 12-22
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Abstract
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- Connexin43 (cx43) forms gap junctions in astrocytes, and these gap junctions mediate intercellular communication by providing transport of low-molecular-weight metabolites and ions. We have recently shown that systemic growth hormone increases cx43 in the brain. One possibility was that local brain insulin-like growth factor-I (IGF-I) could mediate the effect by acting directly on astrocytes. In the present study, we examined the effects of direct application of recombinant human IGF-I (rhIGF-I) on astrocytes in primary culture concerning cx43 protein expression and gap junctional communication (GJC). After 24 hr of stimulation with rhIGF-I under serum-free conditions, the GJC and cx43 protein were analyzed. Administration of 30 ng/ml rhIGF-I increased the GJC and the abundance of cx43 protein. Cell proliferation of the astrocytes was not significantly increased by rhIGF-I at this concentration. However, a higher concentration of rhIGF-I (150 ng/ml) had no effect on GJC/cx43 but increased cell proliferation. Because of the important modulatory role of IGF binding proteins (IGFBPs) on IGF-I action, we analyzed IGFBPs in conditioned media. In cultures with a low abundance of IGFBPs (especially IGFBP-2), the GJC response to 30 ng/ml rhIGF-I was 81%, compared with the average of 25%. Finally, as a control, insulin was given in equimolar concentrations. However, GJC was not affected, which suggests that rhIGF-I acted via IGF-I receptors. In summary, the data show that rhIGF-I may increase GJC/cx43, whereas a higher concentration of rhIGF-I--at which stimulation of proliferation occurred--did not affect GJC/cx43. Furthermore, IGFBP-2 appeared to modulate the action of rhIGF-I on GJC in astrocytes by a paracrine mechanism.
Subject headings
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Neurologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Neurology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Klinisk medicin -- Endokrinologi och diabetes (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Clinical Medicine -- Endocrinology and Diabetes (hsv//eng)
Keyword
- Animals
- Astrocytes
- drug effects
- metabolism
- Cell Communication
- drug effects
- physiology
- Cells
- Cultured
- Connexin 43
- biosynthesis
- genetics
- Gene Expression Regulation
- drug effects
- physiology
- Humans
- Insulin-Like Growth Factor I
- pharmacology
- physiology
- Intercellular Junctions
- drug effects
- metabolism
- Intercellular Signaling Peptides and Proteins
- pharmacology
- physiology
- Rats
- Rats
- Sprague-Dawley
Publication and Content Type
- ref (subject category)
- art (subject category)
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Åberg, N David, ...
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Blomstrand, Fred ...
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Åberg, Maria A I ...
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Björklund, Ulrik ...
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Carlsson, Björn, ...
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Carlsson-Skwirut ...
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Bang, Peter
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Rönnbäck, Lars, ...
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Eriksson, Peter ...
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Aberg, MAL
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- MEDICAL AND HEALTH SCIENCES
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and Clinical Medicin ...
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and Neurology
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MEDICAL AND HEAL ...
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and Clinical Medicin ...
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and Endocrinology an ...
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Journal of neuro ...
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University of Gothenburg
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Karolinska Institutet