Search: onr:"swepub:oai:gup.ub.gu.se/110654" >
Reduced liver injur...
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Oltean, Mihai,1976Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery
(author)
Reduced liver injury and cytokine release after transplantation of preconditioned intestines.
- Article/chapterEnglish2009
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Numbers
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LIBRIS-ID:oai:gup.ub.gu.se/110654
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https://gup.ub.gu.se/publication/110654URI
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https://doi.org/10.1016/j.jss.2008.05.015DOI
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Subject category:ref swepub-contenttype
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Subject category:art swepub-publicationtype
Notes
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BACKGROUND: The postischemic intestine liberates pro-inflammatory mediators (cytokines, lipopolysaccharide [LPS], free radicals) proportional with the local injury that may trigger a systemic inflammatory response and multi-system organ failure. Previously, intestines from donors receiving Tacrolimus revealed improved morphology and abrogated nuclear factor kappa B (NF-kappaB) activation. Because of its pivotal role in inflammation, we investigated if NF-kappaB intragraft inhibition influences the posttransplant inflammatory response and remote organ injury. MATERIALS AND METHODS: Donor Sprague Dawley rats received tacrolimus (0.3 mg/kg) or saline i.v. 6 h before graft harvest. The intestines were preserved for 3 h and then transplanted heterotopically. Hepatic microcirculation was assessed at 20 min, 6 h, 12 h, or 24 h post-reperfusion (postR) using laser-Doppler flowmetry (n = 10/group). Blood pressure measurements and liver sampling were performed at 6, 12, or 24 h postR. Blood samples were obtained at 1, 3, 6, 12, and 24 h postR. Hepatic intercellular adhesion molecule 1 (ICAM-1) expression, caspase-3 and -9 activity, and circulating tumor necrosis factor alpha (TNF-alpha), interleukin-1beta (IL-1beta), IL-6, and LPS were studied. RESULTS: Pretreated graft (PG) recipients had superior cardiovascular parameters at 6 and 12 h postR, while liver perfusion was similar between groups at all time points. Recipients of PG had lower transaminase levels and ICAM-1 liver expression. Liver caspase 3 and 9 activity were similar at 6 and 12 h but increased at 24 h in both groups. At every time point, circulating tumor necrosis factor alph, IL-1beta, and IL-6 were lower in animals receiving PG. LPS was found increased only at the last time point. CONCLUSIONS: Transplantation of tacrolimus-pretreated intestines triggered a milder inflammatory response and decreased liver injury early posttransplantation compared with untreated grafts. Cytokines, but not neutrophils, hypoperfusion, or LPS may underlie the dysfunction.
Subject headings and genre
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MEDICIN OCH HÄLSOVETENSKAP Klinisk medicin Kirurgi hsv//swe
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MEDICAL AND HEALTH SCIENCES Clinical Medicine Surgery hsv//eng
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Animals
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Caspase 3
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metabolism
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Caspase 9
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metabolism
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Cytokines
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secretion
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Immunosuppressive Agents
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therapeutic use
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Interleukin-1beta
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blood
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Interleukin-6
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blood
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Intestines
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transplantation
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Ischemic Preconditioning
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methods
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Lipopolysaccharides
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blood
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Liver
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enzymology
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injuries
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Male
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Rats
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Rats
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Sprague-Dawley
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Reperfusion Injury
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prevention & control
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Tacrolimus
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therapeutic use
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Tumor Necrosis Factor-alpha
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blood
Added entries (persons, corporate bodies, meetings, titles ...)
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Zhu, Changlian,1964Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation(Swepub:gu)xzhuch
(author)
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Mera, SimonaGothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research
(author)
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Pullerits, Rille,1969Gothenburg University,Göteborgs universitet,Institutionen för medicin, avdelningen för reumatologi och inflammationsforskning,Institute of Medicine, Department of Rheumatology and Inflammation Research(Swepub:gu)xpulri
(author)
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Mattsby-Baltzer, Inger,1949Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för infektionssjukdomar,Institute of Biomedicine, Department of Infectious Medicine(Swepub:gu)xmatin
(author)
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Mölne, Johan,1958Gothenburg University,Göteborgs universitet,Institutionen för biomedicin, avdelningen för patologi,Institute of Biomedicine, Department of Pathology(Swepub:gu)xmolnj
(author)
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Hallberg, Eva,1966Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery(Swepub:gu)xhalev
(author)
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Blomgren, Klas,1963Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi, sektionen för klinisk neurovetenskap och rehabilitering,Institutionen för kliniska vetenskaper,Institute of Neuroscience and Physiology, Department of Clinical Neuroscience and Rehabilitation,Institute of Clinical Sciences(Swepub:gu)xblokl
(author)
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Olausson, Michael,1956Gothenburg University,Göteborgs universitet,Institutionen för kliniska vetenskaper, Avdelningen för kirurgi,Institute of Clinical Sciences, Department of Surgery(Swepub:gu)xolami
(author)
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Göteborgs universitetInstitutionen för kliniska vetenskaper, Avdelningen för kirurgi
(creator_code:org_t)
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In:The Journal of surgical research: Elsevier BV154:1, s. 30-71095-86730022-4804
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